Establishment and Verification of An Efficient Virus-induced Gene Silencing System in Forsythia  

作　　者：Jianshuang Shen Weijia Si Yutong Wu Yang Xu Jia Wang Tangren Cheng Qixiang Zhang Huitang Pan 

机构地区：[1]Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding,National Engineering Research Center for Floriculture,Beijing Laboratory of Urban and Rural Ecological Environment of Beijing Municipal Education Commission,College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China [2]Departmentof Landscape Architectrue,Jiyang College of Zhejiang A&F University,Zhuji,Zhejiang 311800,China

出　　处：《Horticultural Plant Journal》2021年第1期81-88,共8页园艺学报（英文版）

基　　金：Thanks for the technical support of Dr.Daqi Fu and Dr.Lanhuan Meng of China Agricultural University.This work was supported by Beijing Municipal Science and Technology Project(Grant No.Z181100002418006);the Fundamental Research Fund for the Central University(Grant No.2015ZCQ-YL-03);the World-Class Discipline Construction and Characteristic Development Guidance Funds for Beijing Forestry University(Grant No.2019XKJS0323).

摘　　要：To understand the functional identification of large-scale genomic sequences in Forsythia,tobacco rattle virus(TRV)-mediated virus-induced gene silencing(VIGS),suitable for the plant,was explored in this study.The results showed that the TRV-mediated VIGS system could be successfully used in Forsythia for silencing the reporter gene FsPDS(Forsythia phytoene desaturase)using stem infiltration and leaf infiltrationmethods.All the treated plants were pruned below the injection site after 7–15 d infection;the FsPDS was silenced and typical photobleaching symptoms were observed in newly sprouted leaves at the whole-plant level.Meanwhile,this system has been successfully tested and verified through virus detection and qRT-PCR analysis.After the optimization,Forsythia magnesium chelatase subunit H(FsChlH)was silenced successfully in Forsythia using this system,resulting in yellow leaveswith decreased chlorophyll content.The system was stable,highly efficient and had greater rapidity and convenience,which made it suitable to study the function of genes related to physiological pathways such as growth and development,and metabolic regulation in Forsythia.

关 键 词：FORSYTHIA Virus-induced gene silencing phytoene desaturase TRV whole-plant level 

分 类 号：S685.24[农业科学—观赏园艺]