UPLC⁃MS/MS法同时测定抗萎平异合剂中7种成分  

Simultaneous determination of seven constituents in Kangwei Pingyi Mixture by UPLC⁃MS/MS

作　　者：李玲慧 李丹 黄汉明 许文[2] 徐伟[2] LI Ling-hui;LI Dan;HUANG Han-ming;XU Wen;XU Wei(Fuzhou Municipal Hospital of Traditional Chinese Medicine,Fuzhou 350001,China;College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福州市中医院,福建福州350001 [2]福建中医药大学药学院,福建福州350122

出　　处：《中成药》2021年第2期321-326,共6页Chinese Traditional Patent Medicine

基　　金：福建省中医重点专科(中药临床药学)(闽卫中医函[2019]262);黄秋云全国名老中医药专家传承工作室(闽卫中医药[2019]21)。

摘　　要：目的建立UPLC⁃MS/MS法同时测定抗萎平异合剂(黄芪、党参、木蝴蝶等)中咖啡酸、毛蕊异黄酮葡萄糖苷、甘草苷、木蝴蝶苷B、党参炔苷、黄芪甲苷、甘草酸的含量。方法该药物50%甲醇提取液的分析采用Waters CORTECS C18色谱柱(2.1 mm×100 mm,1.6μm);流动相乙腈⁃水(含0.1%甲酸),梯度洗脱;体积流量0.25 mL/min;柱温40℃;正离子多反应监测(MRM)模式。以木蝴蝶苷B为指标成分,通过UPLC⁃DAD法测定其含量。结果7种成分在各自范围内线性关系良好(r>0.9990),平均加样回收率97.53%~104.30%,RSD 3.3%~4.3%。3批样品中木蝴蝶苷B含量为1.783~1.811 mg/mL。结论该方法简便准确,可用于抗萎平异合剂的质量控制。AIM To establish a UPLC⁃MS/MS method for the simultaneous content determination of caffeic acid,calycosin⁃7⁃O⁃β⁃D⁃glucoside,liquiritin,baicalein⁃7⁃O⁃gentiobioside,lobetyolin,astragaloside A and glycyrrhizic acid in Kangwei Pingyi Mixture(Astragali Radix,Codonopsis Radix,Oroxyli Semen,etc.).METHODS The 50%methanol extract of this drug was performed on a 40℃thermostatic Waters CORTECS C18 column(2.1 mm×100 mm,1.6μm),with the mobile phase comprising of acetonitrile⁃water(containing 0.1%formic acid)flowing at 0.25 mL/min in a gradient elution manner,and multiple reaction monitoring(MRM)was adopted in positive ion mode.Baicalein⁃7⁃O⁃gentiobioside was taken as an index component,whose content was determined by UPLC⁃DAD.RESULTS Seven constituents showed good linear relationships within their own ranges(r>0.9990),whose average recoveries were 97.53%-104.30%with the RSDs of 3.3%-4.3%.The contents of baicalein⁃7⁃O⁃gentiobioside were 1.783-1.811 mg/mL in three batches of samples.CONCLUSION This simple and accurate method can be used for the quality control of Kangwei Pingyi Mixture.

关 键 词：抗萎平异合剂 化学成分 木蝴蝶苷B UPLC⁃MS/MS UPLC⁃DAD 

分 类 号：R927.2[医药卫生—药学]