依托咪酯对丘脑腹侧连接核神经元活性的影响  

Effect of etomidate on the reuniens neuronal activity

作　　者：王玉龙 薛婷婷 陈永权 WANG Yulong;XUE Tingting;CHEN Yongquan(Department of Anesthesiology,First Affiliated Hospitalof Wannan Medical College,Wuhu Anhui 241000,China)

机构地区：[1]皖南医学院第一附属医院麻醉科,安徽芜湖241000

出　　处：《中南大学学报（医学版）》2021年第1期39-46,共8页Journal of Central South University ：Medical Science

摘　　要：目的:探索依托咪酯对丘脑腹侧连接核神经元活性的影响及机制。方法:在4~5周龄的C57BL/6J小鼠急性脑片上,利用全细胞膜片钳方法检测依托咪酯对丘脑腹侧连接核神经元活性的影响。在电流钳模式下记录丘脑腹侧连接核神经元的电生理特性,然后观察0.5、2.0、8.0μmol/L依托咪酯(分别设为0.5、2.0、8.0μmol/L依托咪酯组)和脂肪乳(脂肪乳组)对丘脑腹侧连接核神经元放电频率和膜电位的影响,记录每个浓度组给药前、给药后和洗脱后的丘脑腹侧连接核神经元放电频率(FB、FD、Fw)及给药前、给药后膜电位(MPB、MPD)。利用木防己苦毒素(picrotoxin,PTX)阻断γ-氨基丁酸A型(gamma-aminobutyric acid Type A,GABAA)受体氯通道,记录给药前、给予亚麻醉浓度(0.5μmol/L)依托咪酯后、继续灌流依托咪酯同时又加入100μmol/L PTX后的丘脑腹侧连接核神经元放电频率(分别为FB.S、FETO和FETO+PTX)。结果:在脂肪乳组中,FB、FD和Fw三者相比,差异无统计学意义(P>0.05)。而在0.5、2.0和8.0μmol/L依托咪酯组中,FD均低于FB,差异均有显著统计学意义(均P<0.01),FW均高于FD,差异均有统计学意义(均P<0.05),并且0.5μmol/L依托咪酯组与8.0μmol/L依托咪酯组相比较,在抑制丘脑腹侧连接核神经元放电频率程度上的差异有统计学意义(P<0.05)。在0.5μmol/L依托咪酯的作用机制实验中,FETO与FB.S相比,差异有显著统计学意义(P<0.01);但FETO+PTX与FB.S.相比,差异无统计学意义(P>0.05)。结论:依托咪酯呈浓度依赖性和可逆性地抑制丘脑腹侧连接核神经元的活性;亚麻醉浓度的依托咪酯主要通过作用于GABAA受体来抑制丘脑腹侧连接核神经元的活性。Objective:To explore the effect of etomidate on the neuronal activity of ventral thalamic reuniens nucleus and the underlying mechanisms.Methods:Whole-cell patch clamp method was used to explore the effect of etomidate on the activity of ventral thalamic reuniens neurons in the acute brain slices obtained from 4-5 weeks old C57BL/6J mice.The electrophysiological characteristics of ventral thalamic reuniens neurons were recorded in the current clamp mode,and then the effects of etomidate(0.5,2.0,8.0μmol/L etomidate groups)and intralipid(intralipid group)on the discharge frequency and membrane potential of ventral thalamic reuniens neurons were recorded.During the experiment,the ventral thalamic reuniens neuron firing rates(RNFRs)were recorded as FB,FD and Fw before,after administration,and after elution;and the membrane potential was recorded as MPB and MPD before,after administration.The chlorine channel of gamma-amino butyric acid Type A(GABAA)receptor was blocked with 100μmol/L picrotoxin(PTX).The RNFRs were recorded as FBS,FETO and FETO+PTX before,after perfusing etomidate with sub-anesthesia concentration(0.5μmol/L)and after perfusing both PTX and etomidate.Results:In the intralipid group,there was no significant difference among the FB,FD and Fw(P>0.05).But in the etomidate groups(0.5,2.0,8.0μmol/L),the FD was less than the FB,there was significant difference(all P<0.01);the Fw was higher than the FD,there was significant difference(all P<0.05).Moreover,there was significant difference in the inhibitory degree of the RNFRs between the 0.5μmol/L etomidate group and the 8.0μmol/L etomidate group(P<0.05).In the experiment to explore the mechanism of etomidate(0.5μmol/L),the FETO was compared with the FBS,there was significant difference(P<0.01);but when the FETO+PTX was compared with the FBS,there was no significant difference(P>0.05).Conclusion:Etomidate can inhibit the activity of ventral thalamic reuniens neurons in concentration-dependent manner,and which is reversible.Etomidate with sub-anesthetic c

关 键 词：依托咪酯 连接核 膜片钳 Γ-氨基丁酸A受体 

分 类 号：R614[医药卫生—麻醉学]