野生欧洲李叶片愈伤组织诱导及增殖培养体系研究  

Study on Callus Induction and Proliferation Culture System of Prunus domestica L.

作　　者：陈曦[1] 颉刚刚 刘柚藓 经建永 马百强 耿文娟[1] CHEN Xi;XIE Ganggang;LIU Youxian;JING Jianyong;MA Baiqiang;GENG Wenjuan(College of Forestry and Horticulture,Xinjiang Agricultural University,Urumqi,Xinjiang 830052)

机构地区：[1]新疆农业大学林学与园艺学院,新疆乌鲁木齐830052

出　　处：《北方园艺》2020年第24期1-7,共7页Northern Horticulture

基　　金：国家自然科学基金资助项目(31560545);国家重点研发计划资助项目(2016YFC0501504);新疆维吾尔自治区园艺重点学科资助项目(2016-10758-3)。

摘　　要：以野生欧洲李叶片为试材,采用组织培养的方法,研究了消毒时间、暗处理时间、基础培养基种类、生长调节剂种类及浓度等影响野生欧洲李叶片愈伤组织诱导及增殖的因素筛选,以期为建立高效野生欧洲李培养体系提供参考依据。结果表明:消毒时间70%酒精处理40 s+0.1%氯化汞6 min处理后叶片的污染率最低为6.67%,死亡率为0%。暗处理28 d时叶片所形成的愈伤组织诱导率最高可达80.5%,叶脉处膨大显著,脱分化形成愈伤组织。叶片在B5培养基中愈伤组织诱导率最大为75.56%。生长调节剂对叶片所形成的愈伤组织诱导率最大可达86.67%。野生欧洲李叶片愈伤组织诱导的最适培养方法为先用70%的酒精消毒40 s,无菌水清洗2~3次,再用0.1%氯化汞溶液浸泡消毒6 min,无菌水清洗4~5次。最佳暗处理时间为14 d。初代培养的较优培养基配方为B5+7.5 mg·L^-1 TDZ+2.5 mg·L^-1 IBA。The leaves of Prunus domestica L.were used as experimental materials in this study.The factors affecting callus induction and proliferation of Prunus domestica L.leaves,such as disinfection time,dark treatment time,type of basic medium,type and concentration of growth regulators and so on,were screened.In order to provide a basis for establishing a highly efficient culture system of Prunus domestica L..The results showed that disinfection time 70% alcohol treatment 40 seconds+0.1% mercuric chloride 6 minutes treatment,the lowest contamination rate of leaves was 6.67%,and the mortality rate was 0%.After 28 days of dark treatment,the callus induction rate of leaves was up to 80.5%,and the leaf veins swelled significantly and dedifferentiated to form calli.The maximum callus induction rate of leaves in B5 medium was 75.56%.The maximum callus induction rate of leaves formed by growth regulators was 86.67%.The optimum culture method for callus induction of Prunus domestica L. leaves was to disinfect 40 seconds with 70% alcohol and wash it with sterile water for 2-3 times.Then soak in 0.1% mercuric chloride solution for 6 minutes and wash it with sterile water for 4-5 times.The best dark treatment time was 14 days.The better medium formula for primary culture was B5+7.5 mg·L^-1 TDZ+2.5 mg·L^-1 IBA.

关 键 词：野生欧洲李 叶片 愈伤组织 组织培养 

分 类 号：S662.3[农业科学—果树学]