大白菜BrTFIIS基因的克隆及表达分析  

Cloning and Expression Analysis of BrTFIIS Gene in Chinese Cabbage

作　　者：齐仙惠[1] 王小军 李改珍[1] 巫东堂[1] 赵军良[1] 王秀英[1] QI Xianhui;WANG Xiaojun;LI Gaizhen;WU Dongtang;ZHAO Junliang;WANG Xiuying(Institute of Vegetables,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030031;Shanxi Vegetable Industry Management Station,Taiyuan,Shanxi 030002)

机构地区：[1]山西省农业科学院蔬菜研究所,山西太原030031 [2]山西省蔬菜产业管理站,山西太原030002

出　　处：《北方园艺》2020年第24期33-38,共6页Northern Horticulture

基　　金：山西省重点研发计划重点资助项目(201703D211006);山西省农业科学院博士研究基金资助项目(YBSJJ1712);山西省农业科学院攻关资助项目(yydzx01);国家大宗蔬菜太原综合试验站资助项目(CARS-23-G-10)。

摘　　要：以大白菜茎尖生长点为研究材料,采用RT-PCR技术,克隆了大白菜BrTFIIS基因,并对其进行生物信息学分析和表达分析,以期为进一步研究大白菜的成花机理提供参考依据。结果表明:BrTFIIS的开放阅读框包含1 008 bp,可编码335个氨基酸;蛋白质分子质量为37.61 kDa,等电点为9.10;系统进化树分析表明BrTFIIS与甘蓝型油菜同源蛋白的亲缘关系最近。荧光定量PCR结果显示,BrTFIIS在大白菜营养生长和生殖生长阶段的茎尖生长点中均有表达,且在春化后表达量明显降低,并在随后的花芽分化进程中维持较低水平。推测BrTFIIS基因可能对大白菜的春化和花芽分化有抑制作用。The stem tip growth point of Chinese cabbage was used as the research material,BrTFIIS gene was cloned by RT-PCR from the shoot apex of Chinese cabbage,and its bioinformatics analysis and expression analysis were carried out,in order to provide reference for further study on the flowering mechanism of Chinese cabbage.The results showed that the open reading frame of BrTFIIS contained 1 008 bp and encoded 335 amino acids.The molecular weight of protein was 37.61 kDa,and the isoelectric point was 9.10.Phylogenetic tree analysis indicated that BrTFIIS had the closest relationship with the homologous protein of Brassica napus.The results of real-time PCR showed that BrTFIIS was expressed in the shoot apex of Chinese cabbage at vegetative and reproductive stages.The expression decreased significantly after vernalization,and maintained a low level in the process of flower bud differentiation.BrTFIIS might have an inhibitory effect on vernalization and flower bud differentiation of Chinese cabbage.

关 键 词：大白菜 BrTFIIS 基因克隆 表达分析 

分 类 号：S634.1[农业科学—蔬菜学]