呼吸道合胞病毒下呼吸道感染病儿外周血微小RNA-145表达与T淋巴细胞亚群的关系  

Relationship between the expression of miR-145 in peripheral blood and T lymphocyte subsets in children with RSV lower respiratory tract infection

作　　者：吴振奎 钟丽花 林则彬 王家文 WU Zhenkui;ZHONG Lihua;LIN Zebin;WANG Jiawen(Department of Paediatrics,Hainan Modern Women and Children Hospital,Haikou,Hainan 571100,China;Department of Paediatrics,Hainan Children's Hospital,Haikou,Hainan 571100,China)

机构地区：[1]海南现代妇女儿童医院儿科,海南海口571100 [2]海南省儿童医院儿科,海南海口571100

出　　处：《安徽医药》2021年第2期361-365,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨呼吸道合胞病毒(RSV)下呼吸道感染肺炎病儿外周血血浆中微小RNA-145(mir-145)表达与外周血单个核细胞(PBMC)中T淋巴细胞亚群的关系。方法选取2017年1月至2018年12月海南现代妇女儿童医院收治的RSV下呼吸道感染肺炎病儿126例作为RSV肺炎组,同期在本院进行体检的健康儿童130例作为健康对照组。RSV肺炎组根据病情严重程度分为轻度组(n=54)、重度组(n=72),并依据重度组是否给予机械通气分为非机械通气组(n=38)、机械通气组(n=34)。利用实时荧光定量PCR(qRT-PCR)检测外周血血浆中mir-145表达水平,使用流式细胞仪及配套试剂盒检测PBMC中T淋巴细胞亚群CD3^+、CD4^+、CD8^+水平,并用MRFlow流式细胞分析软件自动行CD3^+、CD4^+、CD8^+绝对计数,同时计算CD4^+/CD8^+值。结果RSV肺炎组病儿血浆mir-145表达[(1.74±0.48)比(0.99±0.26)]及PBMC中CD8^+水平[(30.75±8.39)%比(21.64±5.92)%]均显著高于健康对照组(P<0.05),PBMC中CD3^+[(59.04±16.12)%比(68.77±18.35)%]、CD4^+水平[(31.27±8.01)%比(46.90±12.84)%]及CD4^+/CD8^+值[(1.02±0.28)比(2.17±0.63)]均明显低于健康对照组(P<0.05)。重度组病儿血浆mir-145表达[(1.87±0.55)比(1.52±0.40)]及PBMC中CD8^+水平[(33.96±9.10)%比(25.34±7.02)%]均明显高于轻度组病儿(P<0.05),PBMC中CD3^+[(57.05±15.83)%比(63.28±17.21)%]、CD4^+水平[(23.78±6.62)%比(38.09±10.14)%]及CD4^+/CD8^+值[(0.70±0.21)比(1.50±0.39)]均显著低于轻度组病儿(P<0.05)。重度RSV肺炎机械通气组病儿血浆mir-145表达[(1.95±0.58)比(1.68±0.45)]及PBMC中CD8^+水平[(34.92±9.18)%比(28.17±7.49)%]均显著高于非机械通气组病儿(P<0.05),PBMC中CD3^+[(54.72±13.53)%比(61.83±16.04)%]、CD4^+水平[(20.45±5.79)%比(34.03±8.85)%]及CD4^+/CD8^+值[(0.59±0.17)比(1.21±0.36)]均明显低于非机械通气组病儿(P<0.05)。RSV肺炎病儿血浆mir-145表达与PBMC中CD3^+水平呈负相关(P<0.05),与PBMC中CD4^+水平呈负相关(P<0.05),与PBMC中CD8^+水�Objective To investigate the relationship between the expression of microRNA-145(mir-145)in peripheral blood plasma and T lymphocyte subsets in peripheral blood mononuclear cells(PBMC)of children with respiratory syncytial virus(RSV)pneumonia.Methods One hundred and twenty-six children with RSV pneumonia admitted to Hainan Modern Women and Children’s Hospital from January 2017 to December 2018 were selected as the RSV pneumonia group,and 130 healthy children who underwent physical examination at the same time were selected as the control group.The children in RSV pneumonia group were assigned into mild group(n=54)and severe group(n=72)according to the severity of the disease,and the children in severe group were assigned into non-mechanical ventilation group(n=38)and mechanical ventilation group(n=34).Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the expression of miR-145 in peripheral blood plasma,the levels of CD3^+,CD4^+,CD8^+of T lymphocyte subsets in PBMC were detected by flow cytometry and kits,the absolute counts of CD3^+,CD4^+,CD8^+were automatically performed by MRFlow flow cytometry software,at the same time,the value of CD4^+/CD8^+was calculated.Results The expression of plasma miR-145[(1.74±0.48)vs.(0.99±0.26)]and CD8^+[(30.75±8.39)%vs.(21.64±5.92)%]in PBMC in RSV pneumonia group were significantly higher than those in control group(P<0.05).The levels of CD3^+[(59.04±16.12)%vs.(68.77±18.35)%],CD4^+[(31.27±8.01)%vs.(46.90±12.84)%]and CD4^+/CD8^+[(1.02±0.28)vs.(2.17±0.63)]in PBMC were significantly lower than those in control group(P<0.05).The levels of plasma miR-145[(1.87±0.55)vs.(1.52±0.40)]and CD8^+[(33.96±9.10)%vs.(25.34±7.02)%]in PBMC in severe group were significantly higher than those in mild group(P<0.05),while the levels of CD3^+[(57.05±15.83)%vs.(63.28±17.21)%],CD4^+[(23.78±6.62)%vs.(38.09±10.14)%]and CD4^+/CD8^+[(0.70±0.21)vs.(1.50±0.39)]in PBMC were significantly lower than those in mild group(P<0.05).The plasma miR-145 expression[(1.95±0.58)vs.(1

关 键 词：呼吸道合胞体病毒感染 肺炎 病毒性 微小RNA-145 T淋巴细胞亚群 

分 类 号：R725.6[医药卫生—儿科]