肉桂醛对大鼠肠系膜动脉的作用和机制  

Effect and mechanism of cinnamaldehyde on rat mesenteric artery

作　　者：程筱涵 白玉华 齐靖[3] 邢妍[3] 陈紫洁 张渝 王瞳 李丽静 郑晓东 CHENG Xiaohan;BAI Yuhua;QI Jing;XING Yan;CHEN Zijie;ZHANG Yu;WANG Tong;LI Lijing;ZHENG Xiaodong(Department of Medicinal Chemistry and Natural Medicinal Chemistry,Harbin Medical University-Daqing,Daqing 163319,Heilongjiang,China;Department of Pharmacology,Harbin Medical University-Daqing,Daqing 163319,Heilongjiang,China;Department of Genetics and Cell Biology,Harbin Medical University-Daqing,Daqing 163319,Heilongjiang,China)

机构地区：[1]哈尔滨医科大学大庆校区药物化学与天然药物化学教研室,黑龙江大庆163319 [2]哈尔滨医科大学 [3]哈尔滨医科大学大庆校区药理教研室,黑龙江大庆163319 [4]哈尔滨医科大学大庆校区遗传学与细胞生物学教研室,黑龙江大庆163319

出　　处：《贵州医科大学学报》2021年第2期152-158,共7页Journal of Guizhou Medical University

基　　金：国家自然科学基金(31500936);黑龙江省自然科学基金(C2017042);2019年中央支持地方高校发展人才培养支持计划-优秀青年人才支持项目;黑龙江省大学生创新创业项目(201810226070,201710226091)。

摘　　要：目的探讨肉桂醛(CA)对大鼠肠系膜动脉的作用和机制。方法 Sprague Dawley(SD)大鼠5只经水合氯醛麻醉后分离出肠系膜组织,取肠系膜动脉制备2~3 mm血管分为对照组、去内皮组(发丝刮除血管内皮)、左旋硝精氨酸甲酯(L-NAME)+吲哚美辛(Indo)组(100 mmol/L L-NAME和10μmol/L Indo孵育30 min)、KCl组(60 mmol/L KCl)及二硫苏糖醇(DTT)组(3 mmol/L DTT孵育30 min)、各组血管段分别用苯肾上腺素(1μmol/L PE)预收缩后,加不同浓度CA(1、10及30μmol/L),采用Myograph血管张力检测系统记录各组大鼠肠系膜动脉张力数值变化;SD大鼠6只水合氯醛麻醉分离肠系膜动脉,分离培养肠系膜动脉内皮细胞(MAECs)和动脉平滑肌细胞(MASMCs),利用细胞免疫荧光实验检测血小板-内皮细胞黏附分子(CD31)和α平滑肌肌动蛋白(α-SMA)表达;将MAECs设置为常氧组(21%Fi O2)和缺氧组(3%Fi O2),分别均给予二甲基亚砜(DMSO)、10及30μmol/L CA处理24 h,采用Western blot实验检测各组MAECs中p-eNOS蛋白的表达;MASMCs分别加DMSO及1、10、30μmol/L CA,采用激光扫描共聚焦显微镜技术检测各组MASMCs中Ca2+浓度的变化。结果1、10及30μmol/LCA均可以舒张PE预收缩后的肠系膜动脉(P<0.001);L-NAME+Indo对1、10、30μmol/L CA舒张肠系膜动脉没有影响(P>0.05);去内皮组1和30μmol/L CA肠系膜动脉血管舒张程度明显低于对应浓度对照组(P<0.001);60 mmol/L KCl组和3 mmol/L DTT组血管舒张程度明显低于对照组血管舒张程度(P<0.001);细胞免疫荧光结果显示,不表达血小板-内皮细胞黏附分子CD31的MAECs数量少于表达CD31的MAECs数量,不表达α平滑肌肌动蛋白α-SMA的MASMCs数量少于表达α-SMA的MASMCs数量;不同浓度CA组大鼠MAECs中p-eNOS蛋白表达水平无差异(P>0.05);不同浓度CA对大鼠MASMCs中Ca2+浓度变化无影响(P>0.05)。结论 CA可以舒张肠系膜动脉,其机制不依赖COX及NO途径,可能与血管内皮完整性、内皮源性超极化因子途径及�Objective To investigate the dilation effect and the underlying mechanism of cinnamaldehyde( CA) on the rat mesenteric artery. Methods Five Sprague Dawley( SD) rats were anesthetized with chloral hydrate,then the mesenteric artery was isolated,2 ~ 3 mm artery ring was prepared and mounted to myography system and divided into the control group,endothelium-denuded group,NG-nitro-L-arginine methyl ester [L-NAME,100 mmol/L,nitric oxide synthase( eNOS)inhibitor] and indomethacin( Indo, 10 μmol/L, cyclooxygenase inhibitor) group, KCl group( 60 mmol/L) and DTT group( Dithiothreitol,3 mmol/L,incubated 30 min). The mesenteric artery was pre-contracted with phenylephrine( PE),the tension was recorded after being treated with different concentrations of CA( 1,10,and 30 μmol/L). The primary culture of mesenteric arterial endothelial cells( MAECs) and mesenteric arterial smooth muscle cells( MASMCs) were generated on isolated mesenteric arteries from six SD rats after being anesthetized with chloral hydrate, cell immunofluorescence experiments were used to identify MAEC and MASMC by using platelet-endothelial cell adhesion molecule-1( CD31) and α-smooth muscle actin( α-SMA) respectively. The MAEC was divided into normoxia( 21% Fi O2) and hypoxia( 3% Fi O2) group and treated with vehicle( dimethyl sulfoxide,DMSO) and CA( 10 and 30 μmol/L) for 24 hours,the expression of p-eNOS was detected by Western blot. The MASMC was treated with DMSO or CA( 1,10,and 30 μmol/L),the intracellular Ca2 +concentration was detected by confocal laser scanning microscope. Results Myography results showed that 1 μmol/L, 10 μmol/L, and 30 μmol/L CA dilated the PEprecontracted mesenteric arteries( P < 0. 001) compared with control groups;L-NAME + Indo did not attenuate the 1 μmol/L,10 μmol/L,and 30 μmol/L CA-induced relaxation( P > 0. 05);in endothelium-denuded group,1 μmol/L and 30 μmol/L CA-induced mesenteric arteries relaxation was lower than that of 1 μmol/L and 30 μmol/L control groups( P < 0. 001);in the KCl( 60 mmol/L) or DTT( 3

关 键 词：肠系膜动脉 二硫苏糖醇 血管舒张 氧化还原 肉桂醛 内皮源性超极化因子 

分 类 号：R966[医药卫生—药理学]