贵州汉族人群21个非CODIS STR基因座的遗传多态性分析  

Genetic polymorphism of 21 non-CODIS STR loci of the Han people in Guizhou

作　　者：张红玲[1] 蔡鹏 熊玲 罗文钰 杨美庆 徐荣兰 王启燕[1] 任峥[1] 季晶焱 黄江[1] ZHANG Hongling;CAI Peng;XIONG Ling;LUO Wenyu;YANG Meiqing;XU Ronglan;WANG Qiyan;REN Zheng;JI Jingyan;HUANG Jiang(Department of Forensic Medicine,Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学法医学院,贵州贵阳550004

出　　处：《贵州医科大学学报》2021年第2期159-164,共6页Journal of Guizhou Medical University

基　　金：贵州省高层次“百”层次创新型人才项目[黔科合平台人才(2020)6012];贵州省高技术产业化示范工程项目[黔发改高技(2016)1345];贵州省科技计划项目[黔科合支撑(2019)2825,黔科合支撑(2020)4Y057];贵州省科技计划基金项目[黔科合(2020)1Y353];大学生创新创业训练项目(DC201710660026,DC20195200903);贵州医科大学一流课程项目(YLKC2019007)。

摘　　要：目的调查贵州汉族人群21个非DNA检索系统(CODIS)的短串联重复序列(STR)基因座的遗传多态性,并评估其在法医学上的应用价值。方法选取301例贵州汉族无关个体血样,采用AGCU21+1荧光标记复合扩增系统对样本进行聚合酶链式反应(PCR),3500型全自动遗传分析仪对PCR扩增产物进行电泳分离,Gene Mapper ID-X 1.3软件进行STR基因分型,Modified-Powerstates软件包获得各基因座的等位基因频率、杂合度(H)、匹配概率(PM)、个体识别率(DP)、非父排除率(PE)、多态信息含量(PIC)、累积个体识别能力(TDP)、累积非父排除率(CPE)及各基因座的Hardy-Weinberg平衡检验,采用Genepop软件对两两基因座进行连锁不平衡分析;同时选择已报道的7个地区汉族人群作为参考,对8个汉族人群基因频率进行主成分分析(PCA),应用MEGA 7.0软件通过Neighbor-Joining(N-J)法构建8个地区汉族人群的系统发育树。结果 21个STR基因座共检测出了157个等位基因,436种基因型,基因频率介于0.001 7~0.584 7,PM介于0.052 6(D19S433)~0.226 8(D1S1627),DP介于0.773 2(D1S1627)~0.947 4(D19S433),PE介于0.272 9(D1S1627)~0.586 7(D22S1045),PIC介于0.533 2(D1S1627)~0.803 0(D19S433);TDP为1-4.645 5×10-20,CPE为0.999 998 822;与已报道的7个汉族群体比较,贵州和湖南汉族人群遗传关系最近,与宁夏汉族份遗传关系最远。结论 21个非CODIS STR在贵州汉族人群具有较好的多态性及个体识别能力,可作为法医学亲权鉴定和个人识别的有效补充。Objective To investigate the genetic polymorphism of 21 non-CODIS STR loci of the Chinese Han people in Guizhou China,and evaluate its applied value in forensic science court.Methods Blood samples collected from 301 unrelated Han people of Guizhou were amplified using AGCU21 + 1 kit,and the amplified products were analyzed by capillary electrophoresis. The STR genotyping results were analyzed by Gene Mapper ID-X 1. 3,then the genetic diversity and forensically relevant population statistics of 21 non-CODIS loci were evaluated in 301 Han people from Guizhou area. In addition,the phylogenetic tree was constructed according to the reported frequency of corresponding loci in other Chinese populations. Results A total of 157 alleles and 436 genotypes from 21 STR loci were found. The gene frequency was between 0. 001 7 ~ 0. 584 7;the random matching rate( PM) was between 0. 052 6( D19 S433) ~ 0. 226 8( D1 S1627);the individual identification power( DP) was between 0. 773 2( D1 S1627) ~ 0. 947 4( D19 S433);the non-paternal exclusion rate( PE) was between 0. 272 9( D1 S1627) ~ 0. 586 7( D22 S1045) and the polymorph information content( PIC) was between 0. 533 2( D1 S1627) ~ 0. 803 0( D19 S433). The total discrimination power( TDP) was 1-4. 645 5 × 10-20,and the cumulative probability of exclusion( CPE) was 0. 999 998 822. In comparison of the genetic relationship among Chinese Han populations in Guizhou and other 7 areas,the Guizhou Han and Hunan Han people were the closest,while the Han people in Ningxia was the furthest. Conclusion The 21 non-CODIS STRs possess high polymorphism and individual recognition ability in Guizhou Han population,and it can be an effective supplement to paternity test and personal identification in forensic applications.

关 键 词：法医遗传学 非DNA检索系统 贵州汉族 遗传多态性 短串联重复序列 亲权鉴定 个体识别 

分 类 号：R89[医药卫生—法医学] DF795.1[医药卫生—临床医学]