基于CdS光电活性材料的无标记凝血酶生物传感器的制备及其性能  

An Unlabeled Thrombin Biosensor Fabricated Based on CdS Photoelectrically Active Material and Its Performance

作　　者：刘月新 代玉芳 金丹露 杨顺景 马纳 张升晖 齐宝平 LIU Yuexin;DAI Yufang;JIN Danlu;YANG Shunjing;MA Na;ZHANG Shenghui;QI Baoping(College of Chemistry and Environmental Engineering,Hubei Minzu University,Enshi 445000,Hubei,China;Department of Rheumatology and Immunology,Minda Hospital of Hubei Minzu University,Enshi 445000,Hubei,China)

机构地区：[1]湖北民族大学化学与环境工程学院,湖北恩施445000 [2]湖北民族大学附属民大医院风湿免疫科,湖北恩施445000

出　　处：《武汉大学学报（理学版）》2021年第1期41-46,共6页Journal of Wuhan University:Natural Science Edition

基　　金：湖北省自然科学基金青年项目(2018CFB247);湖北民族大学高水平科研成果校内培育项目(PY20011);湖北民族大学研究生教育创新项目(MYK2020015);湖北民族大学博士启动基金项目(MY2015B019);湖北省“双一流”建设专项。

摘　　要：基于凝血酶(thrombin,TB)对纤维蛋白原(fibrinogen,FIB)分子中特异性位点的剪切作用,采用光寻址电化学策略,构建了基于CdS光电活性材料的无标记凝血酶生物传感器,实现对凝血酶无标记、简便、快速的检测。通过扫描电子显微镜(SEM)、紫外-可见分光光度法(UV-Vis)、X射线衍射(XRD)及X射线光电子能谱(XPS)对光电活性材料CdS进行分析,并利用循环伏安法(CV)对生物传感器的性能进行表征。结果表明,在最优实验条件下,本文构建的传感器对凝血酶的检出限为6 fmol/L(S/N=3),在0.01~100 pmol/L范围内具有良好的线性关系,为凝血酶的检测提供了一种简便有效的方法。Based on the cleavage of fibrinogen(FIB)specific site by thrombin(TB),a photo-addressable electrochemical strategy was used to construct an unlabeled TB biosensor based on CdS photoelectrically active material to achieve the unlabeled,simple and fast detection.The photoelectrically active material CdS was analyzed by scanning electron microscopy(SEM),UV-visible absorption(UV-Vis),X-ray diffraction(XRD)and X-ray photoelectron spectrometer(XPS),and the performance of the biosensor was characterized by cyclic voltammetry(CV).The results show that under the optimal experimental conditions,the detection limit of the sensor constructed in this paper for TB is 6 fmol/L(S/N=3),and it has a good linear relationship in the range of 0.01-100 pmol/L,which provides a simple and effective method for TB detection.

关 键 词：光电活性材料 凝血酶 无标记 传感器 

分 类 号：O652.1[理学—分析化学]