3种木材DNA提取方法比较研究  

Comparison of Three Methods of DNA Extraction from Wood

作　　者：余敏[1,2] 魏宇创 张浩 刘盛全[1,2] YU Min(School of Forestry&Landscape Architecture,Anhui Agricultural University,Hefei 230036,China;Key Lab of State Forest and Grassland Administration on“Wood Quality Improvement&High Efficient”,Hefei 230036,China)

机构地区：[1]安徽农业大学林学与园林学院,安徽合肥230036 [2]“林木材质改良与高效利用”国家林业和草原局重点实验室,安徽合肥230036

出　　处：《安徽农学通报》2021年第3期6-8,53,共4页Anhui Agricultural Science Bulletin

基　　金：安徽省国际科技合作计划项目(1704e1002226);安徽省自然科学基金项目(1908085QC111)。

摘　　要：以微凹黄檀木材标本为研究对象,分别采用BATB法、PTB法和SDS法对微凹黄檀不同部位木材进行DNA提取试验,并扩增DNA条形码序列ITS2、trnH-psbA和matK,分析不同DNA提取方法对微凹黄檀木材DNA提取和DNA条形码序列扩增的影响。结果表明:采用BATB法从微凹黄檀木材心、边材组织中提取的DNA浓度以及DNA条形码扩增效率均要高于其他2种方法,说明BATB法更适合从长期存储木材中提取DNA。The xylarium wood specimens of Dalbergia retusa were selected from the Wood Collection.Three methods,i.e.,BTAB,PTB and SDS were employed to extract DNA in sapwood and heartwood for further PCR amplification of three potential DNA barcode sequences(ITS2,trnH-psbA,and matK).The effects of different methods on DNA ex⁃traction and PCR for target sequences were analyzed.The results showed that the concentration of DNA and efficacy of PCR using BTAB method was higher than that of PTB and SDS methods from sapwood and heartwood.The BTAB method is more suitable for extracting DNA from long-stored wood.

关 键 词：微凹黄檀 DNA提取 DNA条形码 木材识别 

分 类 号：S781.1[农业科学—木材科学与技术]