水稻芽期耐盐性和耐碱性QTL分析  

QTL Analysis of Salt and Alkali Tolerance at Bud Stage in Rice

作　　者：武琦 张萃雯 李双双[2] 郑洪亮[2] WU Qi;ZHANG Cui-wen;LI Shuang-shuang;ZHENG Hong-liang(Seed Technical Service Center of Heilongjiang Province,Harbin 150008,China;College of Agriculture, Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]黑龙江省种业技术服务中心,黑龙江哈尔滨150008 [2]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《黑龙江农业科学》2021年第2期6-12,共7页Heilongjiang Agricultural Sciences

基　　金：黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2017009);黑龙江省自然科学基金联合引导项目(LH2019C035);国家自然科学基金(31601377,31872884);黑龙江省博士后基金(LBH-Z16188);“东农”学者计划青年才俊项目(17QC02)。

摘　　要：为培育适合盐碱土直播的水稻新品种,本研究利用“小白粳子/空育131”RIL群体(200个株系),以及对盐、碱胁迫条件下水稻芽期的相对根长、相对根数和相对苗高3个耐盐碱相关性状的142个SSR标记遗传连锁图谱进行QTL分析。结果表明:在两种胁迫条件下共检测到11个QTL,与耐盐性和耐碱性相关的QTL分别为4个和7个,分布在第1,2,3,5和12号染色体上,LOD值范围为2.58~4.72,贡献率在7.54%~15.34%。通过比较发现,耐盐性QTL和耐碱性QTL均定位于不同染色体区域,说明水稻芽期耐盐性和耐碱性的遗传机制可能不同。在盐胁迫下,与相对根数相关的qSRRN12和与相对根长相关的qSRRL12定位在相同区间;而在碱胁迫下,与相对苗高相关的qARSH1-1和与相对根数相关的qARRN1定位在相同区间,与相对苗高相关的qARSH1-2和与相对根长相关的qARRL1定位在相同区间,与相对根数相关的qARRN5和相对根长相关的qARRL5定位在相邻区间。这些定位在相同或相邻区间的QTL与相对苗高、相对根数和相对根长性状间的相关性具有高度的一致性。通过比较图谱发现,本研究中的9个QTL与以往不同群体中影响耐盐、碱相关性状的QTL定位在相邻或相同染色体区域,在碱胁迫下检测到的qARSH1-1和qARRN1在前人研究中未见报道,可能是与水稻芽期耐碱性相关的新位点。Rice direct seeding has become an important cultivation method in the northeast saline and alkaline soil rice area in recent years.Creating new rice varieties suitable for directing seeding in salt and alkali soil is necessary for agricultural production.In this study,200 RIL population derived from XBJZ/KY131 and 142 SSR markers were used to analyze the relative seedling height,relative root number and relative root length of rice under salt and alkali stress by QTL.Under the condition of two kinds of stress,we detected 11 QTL,4 for salt resistance and 7 for alkali resistance,distribution in 1,2,3,5,12 chromosomes,LOD value range from 2.58 to 4.72,and contribution rate between 7.54%and 15.34%.By comparison,salt tolerance QTL and alkali tolerance QTL were located at the different chromosome regions,indicating that maybe there were different genetic mechanisms from rice salt tolerance and alkali tolerance at bud stage.qSRRN12 and qSRRL12,which were correlated with relative root number and relative root length,were located at the same interval under salt stress.Under alkali stress,qARSH1-1,which was related to relative seedling height,and qARRN1,which was related to relative root number,were located at the same region,qARSH1-2,which was related to relative seedling height,and qARRL1,which was related to relative root length,were located at the same region,qARRN5 and qARRL5,which were related to relative root number and relative root length,were located in adjacent regions.The correlations between QTLs located at the same or adjacent interval region and the three salt-tolerant traits,such as relative seedling height,relative root number and relative root length,were highly consistent.By comparison mapping,there were 9 QTLs in this study located in adjacent or same chromosome region with the region had been reported,which the QTLs affecting salt tolerance and alkaline tolerance related traits in different populations,but qARSH1-1 and qARRN1 detected under alkali stress had not been reported in previous studies,they m

关 键 词：水稻 芽期 耐盐性 耐碱性 QTL 

分 类 号：S51[农业科学—作物学]