魏氏柠檬酸杆菌淀粉样蛋白编码基因csgA的分子功能  

Molecular functions of amyloid encoding gene csgA in Citrobacter werkmanii

作　　者：王颖思 周刚[1] 彭红[1] 李素娟[1] 谢小保[1] 施庆珊[1] WANG Yingsi;ZHOU Gang;PENG Hong;LI Sujuan;XIE Xiaobao;SHI Qingshan(Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,State Key Laboratory of Applied Microbiology Southern China,Guangdong Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070,China)

机构地区：[1]广东省微生物研究所,广东省科学院,华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广州510070

出　　处：《工业微生物》2021年第1期1-7,共7页Industrial Microbiology

基　　金：广东省科学院发展专项资金项目(Nos.2019GDASYL-0104006和2017GDASCX-0102);国家自然科学基金(No.31770091);广东省自然科学基金(No.2020A151501848);广东省科技计划项目(No.2017B030314045)。

摘　　要：CsgA是淀粉样纤维蛋白(Curli)重要组成部分,用同源重组方法对魏氏柠檬酸杆菌中该蛋白的编码基因csgA进行了敲除,通过生物信息学分析和生长曲线、生物膜、刚果红吸附等实验,发现csgA基因敲除后不影响魏氏柠檬酸杆菌浮游菌的生长,但敲除菌株ΔcsgA的生物膜无法形成,同时,ΔcsgA对羧苄青霉素、庆大霉素、头孢噻肟等的耐药性增强,并且csgA基因也是Ca^2+增强魏氏柠檬酸杆菌生物膜形成的作用位点之一。本研究的结果揭示了csgA基因在魏氏柠檬酸杆菌中具有重要的功能,也为该菌株生物膜的防控提供靶点。CsgA is an important component of amyloid fibrin(curli),so a homologous recombination method was used to knock out its encoding gene csgA from Citrobacter werkmanii.Through bioinformatics analysis,growth curve determination,biofilm formation assay,Congo red absorption experiment and other tests,it was found that the deletion of csgA gene did not affect the planktonic growth of C.werkmanii.However,the biofilms of the knockout strain ofΔcsgA could not be formed.Meanwhile,ΔcsgA exhibited more resistance to carbenicillin,gentamicin and cefotaxime,and the csgA gene was also one of the action sites where Ca^2+enhanced the formation of C.werkmanii biofilm.The results of this study revealed that the csgA gene had diverse functions in C.werkmanii and also provided a target for the control of this strain’s biofilm.

关 键 词：魏氏柠檬酸杆菌 淀粉样蛋白 基因敲除 生物膜 抗药性 

分 类 号：Q933[生物学—微生物学]