Optimization of cryopreservation of pathogenic microbial strains  

作　　者：Ning Guo Qiang Wei Yi Xu 

机构地区：[1]National Pathogen Resource Center(NPRC),Chinese Center for Disease Control and Prevention,Beijing 102206,China [2]Institute of Biothermal Science and Technology,University of Shanghai for Science and Technology,Shanghai 200093,China

出　　处：《Journal of Biosafety and Biosecurity》2020年第2期66-70,共5页生物安全和生物安保杂志（英文）

基　　金：the National Science and Technology Major Project on Important Infectious Diseases Prevention and Control(2018ZX10734404);Key technology research for standardized identification of important pathogens and establishment of relevant reference libraries and the National Natural Science Foundation of China(No.51576132,52076140).

摘　　要：The preservation of pathogenic microbial strains is of great significance for basic research in microbiology and utilization of microbial resources.Appropriate preservation methods can maintain high survival rate and genetic stability of pathogenic microbial strains for a long time.This study summarizes the current status of preservation of strains,discusses the limitations and damaging factors of cryopreservation and prospects for optimization,and explores the mechanism of cryopreservation of strains.Our main objective is to provide a reference for the long-term effective preservation of microbial resources and guarantee of national biosafety.

关 键 词：CRYOPRESERVATION CRYOPROTECTANTS Pathogenic microbial strains 

分 类 号：Q93[生物学—微生物学]