聚能超声-醇盐双水相耦合提取藜麦总黄酮  

Extraction of Total Flavonoids from Quinoa by Polymeric Ultrasound-Alcohol Combined with Two-phase Extraction

作　　者：邹维 崔恩浩 丁岗芯 石峰 高云涛 熊华斌[1] 李晓芬 ZOU Wei;CUI En-hao;DING Gang-xin;SHI Feng;GAO Yun-tao;XIONG Hua-bin;LI Xiao-fen(College of Chemistry and Environment,Yunnan Minzu University,Kunming 650500,Yunnan,China;Joint Research Center for International Cross-border Ethnic Regions Biomass Clean Utilization in Yunnan,Yunnan Minzu University,Kunming 650500,Yunnan,China;National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials,Yunnan Minzu University,Kunming 650500,Yunnan,China)

机构地区：[1]云南民族大学化学与环境学院,云南昆明650500 [2]云南民族大学云南省跨境民族地区生物质资源清洁利用国际联合研究中心,云南昆明650500 [3]云南民族大学生物基材料绿色制备技术国家地方联合工程研究中心,云南昆明650500

出　　处：《食品研究与开发》2021年第4期83-89,共7页Food Research and Development

基　　金：国家自然科学基金(21367025);云南省科技计划项目(2017FD118)。

摘　　要：该文将杆式超声变幅器浸入正丙醇-氯化钠双水相提取体系内,建立一种新型聚能超声-双水相集成提取方法,应用于藜麦总黄酮的提取,研究聚能超声辅助提取条件,采用响应面优化方法获得总黄酮得率和影响因素的多元回归模型,发现醇水体积比是影响提取的显著因素,超声时间、液固比和醇水比3个因素之间存在着明显的交互作用,优化获得最佳提取条件为:超声时间26 min、液固比28∶1(mL/g)、醇水体积比0.9∶1,在最佳条件下,藜麦总黄酮得率为0.361%。A rod-type ultrasonic horn was immersed in a propanol-sodium chloride aqueous two-phase extraction system,and a new type of polymeric ultrasound-two-phase integrated extraction method was established.The conditions of shaped ultrasound-assisted extraction were studied,and the multipleregression model of total flavonoid yield and influencing factors was obtained by using response surfaceoptimization method.It was found that the alcohol-water ratio was a significant factor affecting extraction.There was obvious interaction between the three factors of ultrasound time,liquid-solid ratio and alcohol water volume.The optimal extraction conditions were as follows:ultrasonic time 26 min,liquid-solid ratio 28∶1(mL/g),alcohol water volume 0.9∶1,under the optimum conditions,the extraction rate of flavonoids in quinoa was 0.361%.

关 键 词：藜麦 总黄酮 聚能超声辅助提取 正丙醇-氯化钠双水相 响应面优化 

分 类 号：R284.2[医药卫生—中药学]