杜仲中京尼平苷酸的精制工艺对比  

Optimization of Concentrate Process of Geniposidic Acid from Eucommia ulmoides

作　　者：陈骁鹏[1] 吴芸 叶慧[1] CHEN Xiao-peng;WU Yun;YE Hui(Taizhou Institute for Food and Drug Control,Taizhou 225300,China)

机构地区：[1]江苏省泰州市食品药品检验所,江苏泰州225300

出　　处：《中国现代中药》2020年第12期2080-2084,共5页Modern Chinese Medicine

基　　金：泰州市市场监督管理局质量发展与能力提升项目(202008)。

摘　　要：目的:探索并对比中药杜仲中京尼平苷酸的精制工艺。方法:以京尼平苷酸为指标,在明确跨膜压力差、pH因素影响的基础上,采用Box-Behnken中心组和设计建立数学模型,考察膜孔径、跨膜压力差、pH,优选杜仲提取液的浓缩工艺,并与减压浓缩对比分析。结果:优化京尼平苷酸纳滤浓缩工艺为膜孔径400 Da,跨膜压力差1.40 MPa,pH 6.80,京尼平苷酸平均截留率为(93.7±1.8)%,与理论截留率为95.0%相近,且明显高于减压加热浓缩工艺。结论:Box-Behnken响应面法设计优化的杜仲中京尼平苷酸的精制工艺,工艺高效可行,可以避免热处理引起的成分转化损失。Objective:To explore the optimum concentrate process of geniposidic acid from Eucommia ulmoides.Methods:Based on single factor test,nanofiltration membrane pore size,transmembrane pressure difference and pH were selected as independent variables,and the rejection of geniposidic acid was used as dependent variables.Response surface methodology of Box-Behnken Design was applied to optimize the nanofiltration concentrate process of geniposidic acid from E.ulmoides.Meanwhile,the concentrate efficiency of nanofiltration technology was compared with decompressing concentration.Results:The optimum nanofiltration concentrate conditions were nanofiltration membrane pore size 400,transmembrane pressure difference 1.4 MPa and pH 6.80,the rejection of geniposidic acid was(93.7±1.8)%under the optimum conditions.The concentrate efficiency of nanofiltration was significantly better than that of the decompressing concentration.Conclusion:The geniposidic acid from E.ulmoides has been concentrated efficiently by the above optimized technology.The transformation loss of heat-sensitive ingredients can be avoided by nanofiltration technology.

关 键 词：杜仲 京尼平苷酸 纳滤 减压浓缩 响应面法 

分 类 号：R282.71[医药卫生—中药学] R284.2[医药卫生—中医学]