大豆KTI1基因表达方式分析及其启动子预测  

Analysis of Expression Patterns of KTI1 Gene in Soybean and Its Promoter Prediction

作　　者：赵艳[1] 王珏 刘阔成 孙天国[1] Zhao Yan;Wang Jue;Liu Kuocheng;Sun Tianguo(Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,College of Life Science and Agroforestry,Qiqihar University,Qiqihar,161006)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,齐齐哈尔161006

出　　处：《分子植物育种》2021年第2期380-384,共5页Molecular Plant Breeding

基　　金：齐齐哈尔大学研究生创新科研项目(YJSCX2019052)资助。

摘　　要：为明确大豆胰蛋白酶抑制剂基因(KTI1)在不同逆境、ABA条件下及大豆不同组织中的表达方式,本研究采用实时荧光定量PCR技术检测,结果表明KTI1基因受低温、盐、干旱和ABA诱导,分别在处理10、5、24和1 h时获得诱导最高值,相对表达量分别是未处理的288.65,274.35,136.04和56.48倍;KTI1基因在大豆种子中的表达量相对较高,叶中的表达量为1时,种子中的表达量为8.70。根据大豆基因组序列,扩增KTI1基因ATG上游启动子序列1 831 bp;生物信息学预测分析表明启动子中存在多种常出现在逆境胁迫诱导型启动子中的顺式调控元件,推测大豆KTI1基因启动子可能是受低温、盐、干旱和ABA诱导的诱导型启动子。本研究为进一步研究和应用大豆KTI1基因及其启动子提供理论基础。In order to clarify the expression pattern of soybean trypsin inhibitor gene(KTI1)in different stress,ABA conditions and different tissues of soybean,the Quantitative Real-Time PCR method was used in this study.The results showed that KTI1 gene was induced by low temperature,salt,drought and ABA,inducing high respectively in the treatment of 10,5,24 and 1 h.The relative expression levels were 288.65,274.35,136.04 and 56.48 times higher than that of untreated.The expression of soybean KTI1 gene in soybean seeds was relatively high,and when the expression level was 1 in leaves,the expression levels in seeds was 8.70.According to soybean genome sequence,1831 bp upstream promoter sequence of KTI1 gene was amplified.Bioinformatics predictive analysis showed that there were a variety of cis-regulatory elements in promoter that often appeared in stressinduced promoters.It was speculated that soybean KTI1 gene promoter might induce by low temperature,salt,drought and ABA.This study provides a theoretical foundation for further study and application of soybean KTI1 gene and its promoter.

关 键 词：大豆 KTI1基因 启动子 

分 类 号：S565.1[农业科学—作物学]