三个大果型番茄花药愈伤组织诱导分析  

Anther Callus Induction Analysis of Three Large Fruit Tomato

作　　者：罗佳[1] 杭晓宁[2] 汝学娟[1] 尹贤贵[1] 谢树章[3] 潘光辉[1] Luo Jia;Hang Xiaoning;Ru Xuejuan;Yin Xiangui;Xie Shuzhang;Pan Guanghui(Vegetables and flowers research institution of Chongqing Academy of Agricultural Sciences,Chongqing,401329;Agricultural resource and environment research institution of Chongqing Academy of Agricultural Sciences,Chongqing,401329;Chongqing Key Laboratory of Adversity Agriculture,Biotechnology Research Institution of Chongqing Academy of Agricultural Sciences,Chongqing,401329)

机构地区：[1]重庆市农业科学院蔬菜花卉研究所,重庆401329 [2]重庆市农业科学院农业资源与环境研究所,重庆401329 [3]重庆市农业科学院生物技术研究所,逆境农业研究:重庆市市级重点实验室,重庆401329

出　　处：《分子植物育种》2021年第2期576-583,共8页Molecular Plant Breeding

基　　金：重庆市科研机构绩效激励引导专项项目(cstc2019jxjl80006);重庆市技术创新与应用发展专项重点项目(cstc-2019jscx-gksbX0149);重庆市基本科研业务费计划项目(2016cstc-jbky-00511)共同资助。

摘　　要：本研究以3个大果型番茄杂交种F1代(‘1401’,‘1403’,‘卡莱’)的花药为试验材料,对其花药愈伤组织诱导的影响因素进行初步研究。结果表明:大果型番茄花药小孢子发育时期与花蕾长度以及花药长度呈正相关。较适番茄花蕾消毒方法为用70%酒精对番茄花蕾进行表面消毒30 s,然后再用加了0.1%吐温20的15%NaClO消毒7 min。3个供试番茄品种均能诱导产生愈伤组织,但不同番茄品种的愈伤组织诱导率不同。其中,‘1401’番茄的愈伤组织诱导率最高,为19.67%。诱导培养基中添加30 g/L蔗糖能促进愈伤组织的产生,生长素与细胞分裂素浓度比为1:2有利于愈伤组织的产生。较适愈伤组织诱导培养基为(murashige and skoog medium,MS)+0.5 mg/L IAA+1 mg/L 6-BA+30 g/L蔗糖+7 g/L琼脂粉。该研究为获得大果型番茄多倍体材料提供了技术支撑。The anthers of three large fruit tomato F1 hybrids(’1401’,’1403’,’Kale’)were used as experimental materials to preliminarily study the influencing factors of anther callus induction.The results showed that there was a positive correlation between the microspore developmental stage and the length of flower bud or anther.The appropriate sterilizing method of tomato flower bud was to surface-sterilize the tomato flower bud with 70%alcohol for 30 s,then sterilize it with 15%NaClO with 0.1%Tween 20 for 7 min.Callus could be induced from three tested tomato varieties,but the callus induction rate of different tomato varieties was different.Among them,’1401’tomato had the highest callus induction rate(19.67%).The induction medium with 30 g/L sucrose can promote the production of callus,the ratio of auxin to cytokinin was 1:2,which was beneficial to callus production.The appropriate medium for callus induction was MS+0.5 mg/L IAA+1 mg/L 6-BA+30 g/L sucrose+7 g/L agar powder.This study provided technical support for obtaining polyploidy materials of large fruit tomato.

关 键 词：大果型番茄 花药 愈伤组织 诱导 

分 类 号：S641.2[农业科学—蔬菜学]