烟草航天诱变突变体叶形遗传分析及基因定位  

Leaf Shape Genetic Analysis and Gene Mapping of Tobacco Space Mutation Mutant

作　　者：周世奇 罗琳 程立锐[1] 潘旭浩[1] 胡海洲[1] 刘杨 尹鹏嘉[1] 张玉[1] 罗成刚[1] Zhou Shiqi;Luo Lin;Cheng Lirui;Pan Xuhao;Hu Haizhou;Liu Yang;Yin Pengjia;Zhang Yu(Key Laboratory ofTabacco Genetic Improvement and Biotechnology,Tobacco Research Institute of CAAS,Qingdao,266101;Liangshan Tobacco Company of Sichuan Province,Xichang,615000;Sichuan Province Tobacco Research Institute,Chengdu,610041)

机构地区：[1]中国农业科学院烟草研究所,中国农业科学院烟草遗传改良与生物技术重点开放实验室,青岛266101 [2]四川省烟草公司凉山州公司,西昌615000 [3]中国烟草总公司四川省公司,成都610041

出　　处：《分子植物育种》2021年第2期584-590,共7页Molecular Plant Breeding

基　　金：四川省烟草专卖局科技重点项目(SCYC201701,SCYC201501)资助。

摘　　要：利用航天诱变方法获得烟草品种NC89的突变体NC89-M,与野生型相比,NC89-M叶形呈宽椭圆,叶面褶皱,叶缘呈锯齿状。为明确烟草航天诱变突变体NC89-M叶形突变机理,挖掘突变体叶形突变基因,利用NC89-M与烟草品系2014-168杂交获得F2群体,对F2群体遗传分析表明突变体叶形性状受1对显性主效基因控制。通过混合集团分离法,确定SSR标记PT60795与叶形性状连锁。利用已有的突变体基因组序列信息,开发InDel标记最终将突变基因nc89m定位于标记PT60795与InDel5之间,遗传距离分别为2.9 cM和4.4 cM。在该区间内发现在叶片中高表达的基因Nitab4.5_0000008g0880.1编码区第1 294个核苷酸发生突变,由腺嘌呤突变为胞嘧啶,其编码氨基酸由酪氨酸突变为天冬氨酸,因此将其作为nc89m的候选基因。本研究为Nitab4.5_0000008g0880.1的基因克隆及功能验证打下基础。The mutant NC89-M was mutanted by tobacco NC89 using aerospace mutagenesis.Compared with the wild type,the leaf shape of the NC89-M is broadly elliptical,the leaf surface is folded,and the leaf edge is jagged.To clarify the mechanism of tobacco leaf-shaped space mutant NC89-M and excavate leaf-shape mutant gene.The F2 population was obtained by hybridization of NC89-M with the tobacco strain 2014-168.Genetic analysis among F2 population indicated that the mutant leaf shape was controlled by one pair of dominant genes.The SSR marker PT60795 was determined to be linked to the leaf shape trait by bulked segregant analysis.According to the mutant genome sequence,anew developed InDel marker mapped the mutated gene nc89 m between the markers PT60795 and InDel5 with genetic distances of 2.9 c M and 4.4 cM,respectively.In this interval,the 1294 thnucleotide of the coding region of the gene Nitab4.50000008 g0880.1 was found to be mutated from adenine to cytosine,and its encoded amino acid was mutated from tyrosine to aspartic acid.The gene expression is higher in the leaf,so it is used as a candidate gene for nc89 m.This study laid the foundation for the cloning and functional verification of the gene Nitab4.50000008 g0880.1.

关 键 词：烟草(Nicotiana tabacuma) 叶形 基因定位 候选基因 

分 类 号：S572[农业科学—烟草工业]