长链非编码RNA HOXA簇反义RNA2在重度子痫前期患者胎盘组织中的表达及其作用  

Expression and role of antisense RNA2 of long non-coding RNA HOXA cluster in placental tissue of patients with severe preeclampsia

作　　者：盛莹[1] 钟黎黎[1] 杨春芬 郭江虹[1] 阳双健 SHENG Ying;ZHONG Li-li;YANG Chun-fen;GUO Jiang-hong;YANG Shuan-jian(Department of Obstetrics,First Affiliated Hospital of Nanhua University,Hengyang 421001,Hunan,China)

机构地区：[1]南华大学附属第一医院产科,衡阳421001

出　　处：《医学研究生学报》2021年第2期171-176,共6页Journal of Medical Postgraduates

摘　　要：目的长链非编码RNA HOXA簇反义RNA2(HOXA-AS2)在子痫前期患者胎盘组织中的表达水平及其对子痫前期病理机制的影响,目前还尚不清楚。文中旨在探讨重度子痫前期(SPE)患者胎盘组织中HOXA-AS2的表达水平及其对滋养细胞上皮-间质转化(EMT)的影响。方法选取2017年2月至2019年4月在南华大学附属第一医院妇产科42例住院行剖宫产分娩的SPE患者。根据发病孕龄不同,将孕龄≤34孕周的SPE孕妇纳入早发型SPE组(n=19),将孕龄>34孕周的SPE孕妇纳入晚发型SPE组(n=23)。同时,选择同期行剖宫产分娩的30例正常妊娠晚期孕妇作为对照,其中14例因宫颈机能不全、胎膜早破、前置胎盘等病因而终止妊娠者作为早发型对照组,16例因胎儿窘迫、胎位异常、脐带绕颈等而终止妊娠者作为晚发型对照组。采用qRT-PCR检测各组胎盘组织中HOXA-AS2表达水平。将HOXA-AS2过表达质粒(pcDNA3.1-HOXA-AS2)及其空载质粒(Vector)转染至滋养细胞HTR-8/SVneo中,设置空白对照组(不做任何处理)、空载质粒组(转染空载质粒)和过表达组(转染pcDNA3.1-HOXA-AS2质粒)。qRT-PCR检测细胞中HOXA-AS2表达水平,CCK-8检测细胞增殖活性,Transwell检测细胞侵袭和迁移能力,Western blot检测EMT及Wnt/β-catenin信号通路相关蛋白表达水平。结果与早发型SPE组胎盘组织中HOXA-AS2表达水平比较,早发型对照组、晚发型SPE组及晚发型对照组明显增高(P<0.001)。过表达组细胞中HOXA-AS2表达水平(41.28±4.01)显著高于空白对照组(1.00±0.03)、空载质粒组(1.04±0.04),差异有统计学意义(P<0.01)。过表达组HTR-8/SVneo细胞侵袭和迁移能力较空白对照组、空载质粒组显著增强(P<0.05),Vimentin、N-cadherin、β-catenin、Snail1蛋白表达水平显著增加(P<0.05),而E-cadherin和p-GSK3β等蛋白表达水平显著降低(P<0.05)。结论HOXA-AS2在早发型SPE胎盘组织中低表达,HOXA-AS2过表达可增强HTR-8/SVneo细胞侵袭和迁移能力,�Objective The expression level of antisense RNA2(HOXA-AS2)of long non-coding RNA HOXA in placental tissues of patients with preeclampsia and its effect on the pathological mechanism of preeclampsia are still unclear.This study aims to investigate the expression level of HOXA-AS2 in placental tissue of patients with severe preeclampsia(SPE)and its effect on trophoblast epithelio-mesenchymal transformation(EMT).Methods A total of 42 SPE patients who received cesarean section delivery in the Department of Gynecology and Obstetrics of the First Affiliated Hospital of the University of South China from February 2017 to April 2019 were selected.According to different gestational age at onset,SPE pregnant women with gestational age≤34 weeks were included in the early onset SPE group(n=19),and those with age>34 weeks in the late onset SPE group(n=23).At the same time,30 cases of pregnant women in the third trimester of pregnancy who underwent cesarean section were selected as the control group.14 patients who terminated pregnancy due to cervical insufficiency,premature rupture of membranes,placenta previa and other diseases were used as the early-onset control group;16 patients who terminated their pregnancies due to fetal distress,abnormal fetal position or umbilical cord around the neck were used as the late-onset control group.The expression level of HOXA-As2 in the placental tissues of each group was detected by qRT-PCR.The HOXA-AS2 overexpressing plasmid(pcDNA3.1-HOXA-AS2)and its Vector were transfected into trophoblast cells HTR-8/SVneo,and the blank control group(without any treatment),no-load plasmid group(transfected no-load plasmid)and overexpression group(transfected pcDNA3.1-HOXA-AS2 plasmid)were set.QRT-PCR was used to detect the expression of HOXA-AS2;CCK-8 was used to detect cell proliferation;Transwell was used to detect cell invasion and migration;and Western blot was used to detect the expression of EMT and Wnt/β-catenin signaling pathway related proteins.Results Compared with early onset SPE group,H

关 键 词：HOXA簇反义RNA2 子痫前期 滋养细胞 上皮-间质转化 

分 类 号：R714[医药卫生—妇产科学]