尿液病毒载量检测对先天性巨细胞病毒感染诊断及病情评估的价值分析  

The value of urine viral load test in diagnosis and evaluation of congenital cytomegalovirus infection

作　　者：邱锐琴[1] 吴小波[2] 杜志云[1] 曹沐琳[1] 乔木[1] 姚文秀[1] QIU Rui qin;WU Xiao-bo;DU Zhi-yun(Department of Neonatology of Qinhuangdao First Hospital,Qinhuangdao 066000.China;Department of Pediatrics of Qinhuangdao First Hos pital,Qinhuangdao 066000,China)

机构地区：[1]秦皇岛市第一医院新生儿科,河北秦皇岛066000 [2]秦皇岛市第一医院儿一科,河北秦皇岛066000

出　　处：《中国实验诊断学》2021年第1期43-46,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨尿液病毒载量检测对先天性巨细胞病毒(Human Cytomegalovirus,HCMV)感染诊断及病情评估的价值。方法选择2016年6月至2019年6月期间秦皇岛市第一医院收治的82例HCMV感染患儿作为研究对象,按照HCMV病毒载量检测结果中位数(1.13×104copy/mL),将其分为高病毒载量组(n=38)与低病毒载量组(n=44),采用PCR仪对HCMV病毒载量进行检测。比较高、低病毒载量组患儿临床特征发生情况,高、低病毒载量组患儿生化指标水平,高、低病毒载量组患儿临床转归情况。结果 (1)高、低病毒载量组患儿咳嗽、黄疸、肝脏肿大及脾脏肿大发生率差异均无统计学意义(P均>0.05),高病毒载量组胆汁淤积及听力损失发生率均分别显著高于低病毒载量组(P均<0.05);(2)两组患儿除了ALT、AST及CRP水平差异均无显著统计学意义外,其余各项指标(TBA、LD、LDH及CK-MB)水平,高病毒载量组显著高于低病毒载量组(P均<0.05);(3)高病毒载量组听力损失及住院时间均分别显著高于低病毒载量组(P均<0.05)。结论尿液HCMV病毒载量与患儿疾病严重程度呈正比,其对HCMV病毒感染患儿的临床诊断以及病情评估具有一定的临床价值。Objective To study the value of urine viral load detection in diagnosis and prognosis of Human Cytomegalovirus(HCMV)infection.Methods 82 children with HCMV infection admitted to our hospital from June 2016 to June 2019 were selected as research objects.According to the median of HCMV viral load detection results(1.13×10~4 copy/mL),they were divided into a high viral load group(n=38)and a low viral load group(n=44),and THE HCMV viral load was detected by PCR instrument.The clinical characteristics of the children with high and low viral loads,the biochemical indicators of the children with high and low viral loads,and the clinical outcome of the children with high and low viral loads were compared.Results (1)there were no statistically significant differences in the incidence of cough,jaundice,hepatomegalia and splenomegalia between the high and low viral load groups(all P>0.05).The incidence of cholestasis and hearing loss in the high viral load group was significantly higher than that in the low viral load group(all P<0.05).(2)Except for no statistically significant difference in ALT,AST and CRP levels between the two groups,the levels of other indicators(TBA,LD,LDH and CK-MB)were significantly higher in the group with high viral load than in the group with low viral load(All P<0.05).(3)The hearing loss and length of hospital stay in the group with high viral load were significantly higher than those in the group with low viral load(All P<0.05).Conclusion Urine HCMV viral load is in direct proportion to the severity of disease in children,and it has a certain clinical value in the clinical diagnosis and disease assessment of children with HCMV virus infection.

关 键 词：尿液病毒载量 先天性巨细胞病毒感染 诊断 病情评估 

分 类 号：R446.12[医药卫生—诊断学]