中国明对虾钙调磷酸酶基因calcineurin B表达量的遗传变异分析  

An analysis of genetic variation in calcineurin B gene expression in Fenneropenaeus chinensis

作　　者：李波波 孔杰[2,3] 卢霞 孙犁[1,2] 隋娟 刘绵宇 罗坤[2,3] 孟宪红 陈宝龙 曹宝祥[2,3] 刘宁 栾生[2,3] LI Bobo;KONG Jie;LU Xia;SUN Li;SUI Juan;LIU Mianyu;LUO Kun;MENG Xianhong;CHEN Baolong;CAO Baoxiang;LIU Ning;LUAN Sheng(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院黄海水产研究所,农业农村部海洋渔业可持续发展重点实验室,山东青岛266071 [3]青岛海洋科学与技术国家实验室,海洋渔业科学与食物产出过程功能实验室,山东青岛266071

出　　处：《中国水产科学》2021年第1期12-18,共7页Journal of Fishery Sciences of China

基　　金：国家自然科学基金面上项目(31572616);联合基金项目(U1706203);现代农业产业技术体系专项资金项目(CARS-48);国家重点研发计划项目(2018YFD0901301);山东省农业良种工程项目(2019LZGC014);国家自然科学基金青年科学基金项目(31602151).

摘　　要：为了评估中国明对虾(Fenneropenaeus chinensis)核心育种群体calcineurin B基因(FcCN-B)表达量的遗传变异水平,从2018年构建的G13群体中随机选取32个家系,每个家系随机选择20尾个体,分别放入20个网箱中(每个网箱包括32尾虾),限制饲料投喂量,利用Real-time PCR方法检测每尾虾神经节组织中FcCN-B基因的表达量。结果表明,FcCN-B基因在个体水平上表达明显呈正偏态分布,均值(8.25)大于中位数(6.95),表明存在极大值个体;极差为43.48,最大值是最小值的44.48倍,个体间表达量的变异系数为66.55%。家系间FcCN-B表达量的变异系数相对较小(19.31%),最大值仅为最小值的2.20倍。单因素方差分析表明,FcCN-B基因的表达量在家系水平上差异显著(P<0.05)。利用个体动物模型结合系谱信息,获得FcCN-B基因表达量的遗传力估计值为0.60±0.13。这表明其在育种群体中具有较高的遗传变异水平,遗传改良的潜力大。研究结果为解析中国明对虾竞争行为的分子机理、改良其竞争性提供了重要的参考数据。This study aimed to evaluate the effect of genetic variation on expression of the calcineurin B gene(FcCN-B)in a breeding population of Fenneropenaeus chinensis.Thirty-two full-sib families of the G13 generation were randomly selected in 2018,and one individual from each family was randomly selected and rehomed to new cages(each cage contained 32 shrimps).The amount of feed was limited,and real-time PCR was used to detect the expression of the FcCN-B gene in the ganglion tissue of each shrimp.The results showed that the expression of the FcCN-B gene at the individual level had a positive skew distribution.The higher value of the mean(8.25)compared to the median(6.95)indicates the presence of individuals with extremely high expression.The maximum expression of individuals was 44.48 times the minimum expression of individuals,and the range was 43.48.The variation coefficient of FcCN-B expression at the individual level was 66.55%,whereas it was relatively small(19.31%)at the family level.The maximum expression was only 2.20 times the minimum expression at the family level.One-way analysis of variance showed that there were significant differences in the expression level of the FcCN-B gene between families(P<0.05).Heritability for FcCN-B gene expression,estimated using the animal models and their pedigrees,was 0.60±0.13.This indicates that there was a high level of genetic variation in the nuclear breeding population,and great genetic gain may be obtained.The results provide reference data to investigate the molecular mechanism of the competitive behavior and improve competitive ability in F.chinensis.

关 键 词：中国明对虾 钙调神经磷酸酶B亚基基因 基因表达 遗传力 遗传变异 

分 类 号：S965[农业科学—水产养殖]