恒温高温调节对赤点石斑鱼幼鱼的生长、性腺发育以及gnrh、fshβ和lhβ基因表达的影响  

Effects of regulation of constant high water temperature on growth and gonadal development,as well as gnrh,fshβ,and lhβgene expression in the juvenile red spotted grouper Epinephelus akaara

作　　者：徐文刚 唐永政[1] 张建柏 真鍋颯一郎 征矢野清 邹华锋 XU Wengang;TANG Yongzheng;ZHANG Jianbai;MANABE Soichiro;SOYANO Kiyoshi;ZOU Huafeng(School of Ocean,Yantai University,Yantai 264005,China;Yantai Institute of Marine Economy,Yantai 264003,China;Institute for East China Sea Research,Nagasaki University,Nagasaki 851-2213,Japan;National Demonstration Center for Experimental Fisheries Science Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]烟台大学海洋学院,山东烟台264005 [2]烟台市海洋经济研究院,山东烟台264003 [3]长崎大学环中国东海环境资源研究所,日本长崎851-2213 [4]上海海洋大学水产科学国家级实验教学示范中心,水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《中国水产科学》2021年第1期19-28,共10页Journal of Fishery Sciences of China

基　　金：山东省农业重大应用技术创新项目(SD2019YY003);日本学术振兴会(JSPS)研究项目(26660173);烟台大学博士科研启动基金(HX20B29).

摘　　要：为了解恒定高温对赤点石斑鱼(Epinephelus akaara)生长和繁殖的影响,以孵化后120~420日龄的幼鱼为研究对象,对性腺发育以及与繁殖相关的基因gnrh、fshβ和lhβ表达进行了研究。对照组为自然水温(13.2~27.1℃)饲养,实验组为26℃恒温饲养。每隔30 d或40 d采样1次,比较两组中鱼的体长、体重、性腺指数(GSI)、性腺发育情况以及gnrh、fshβ和lhβ基因表达的差异。结果表明,从240日龄开始,实验组鱼的体长和体重均显著高于对照组(P<0.05)。实验结束时,实验组体长约为对照组的1.3倍,体重约为对照组的2倍。对照组GSI一直维持在0.25%以下的低值,实验组GSI在330日龄前处于0.4%以下,但在360日龄时出现GSI 1%以上的个体。在360、390和420日龄的个体中,实验组GSI显著高于对照组(P<0.05)。实验结束时,对照组中未检测到具有卵黄形成期卵母细胞的个体,但实验组在330、360和390日龄出现了具有卵黄成熟期卵母细胞的个体。对照组和实验组间幼鱼脑中gnrh基因表达在各年龄段均无显著性差异(P>0.05),但脑垂体中fshβ表达在360、390和420日龄,lhβ在390和420日龄时,实验组均显著高于对照组(P<0.05)。研究表明,26℃恒温调节不仅能促进赤点石斑鱼幼鱼体长、体重和GSI的增加,同时也能显著提高脑垂体中fshβ和lhβ基因含量,从而加速性腺发育成熟。The growth,development,and reproduction of teleosts are significantly affected by external environmental factors and the functioning of the endocrine system.To understand the effects of regulation with constant water temperature on fish growth and gonadal development,as well as gnrh,fshβ,and lhβreproduction-related gene expression,the juvenile red spotted grouper Epinephelus akaara,aged 120 to 420 days post hatching(dph),was used as the target species.Fish in the control group were fed at natural water temperature,whereas those in the experimental groups were fed at a constant water temperature of 26℃.Fish were sampled every 30 or 40 days.The body length(BL),body weight(BW),gonadal somatic index(GSI),gonadal development frequency,and expression of gnrh,fshβ,and lhβgenes were measured,calculated,and compared between the two groups.Results showed that from 240 dph,both the BL and BW in the experimental group were significantly higher than those in the control group(P<0.05).At the end of the trial,the BL and BW in the experimental group were 1.3 and 2 times those of the control group,respectively.The GSI in the control group was maintained below 0.25%.In the experimental group,the GSI was maintained below 0.4%before 330 dph but reached 1%in some individuals at 360 dph.At 360,390,and 420 dph,the GSI in the experimental group was significantly higher than that in the control group(P<0.05).At the end of the trial,vitellogenic oocytes were not detected in the control group but were detected at 330,360,and 390 dph in the experimental group.There were no significant differences in gnrh gene levels in the brain at each stage between the control and experimental group(P>0.05).However,in the pituitary,the fshβgene levels at 360,390,and 420 dph and lhβgene levels at 390 and 420 dph in the experimental group were significantly higher than those in the control group(P<0.05).These results suggest that the regulation of constant water temperature at 26℃may not only promote an increase in BL,BW,and GSI but also significa

关 键 词：赤点石斑鱼 幼鱼 恒温高温 生长 性腺发育 GNRH FSHΒ lhβ 

分 类 号：S965[农业科学—水产养殖]