中期及长期模拟失重对大鼠颈总动脉钙化的影响  

Mid-term and long-term simulated microgravity causes calcification of common carotid artery in rats

作　　者：刘焕 茹凝玉 吕强 郭池华 郭玉芳 王爽 苏兴利 马进 Liu Huan;Ru Ning-Yu;Lv Qiang;Guo Chi-Hua;Guo Yu-Fang;Wang Shuang;Su Xing-Li;Ma Jin(Department of Basic Medicine,Xi'an Medical University,Xi'an 710021,China;Department of Aerospace Physiology,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]西安医学院基础医学部,西安710021 [2]空军军医大学航空航天生理学教研室,西安710032

出　　处：《解放军医学杂志》2021年第1期1-6,共6页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(81601637,81800211);陕西省重点研发计划项目(2020SF-156);陕西省自然科学基础研究计划项目(2016JQ8001);西安医学院博士科研启动基金(2017DOC16)。

摘　　要：目的探讨中期(4周)及长期(8周)模拟失重对大鼠颈总动脉钙化的影响。方法采用尾部悬吊后肢去负荷模型模拟微重力对心血管系统的影响。选取雄性SD大鼠75只,随机分为对照组(n=25)、4周尾部悬吊组(HU4周组,n=25)和8周尾部悬吊组(HU8周组,n=25)。模拟失重完成后,分离并收集大鼠颈总动脉组织,采用钙含量测定和茜素红染色方法检测钙盐沉积,进行碱性磷酸酶(ALP)活性测定,通过Western blotting和实时荧光定量PCR检测Runt相关转录因子2(Runx2)、肌节同源盒基因同系物2(Msx2)、骨形态发生蛋白2(BMP2)、骨钙素(osteocalcin)、平滑肌22α(SM22α)蛋白和mRNA的表达水平。结果与对照组比较,4周和8周模拟失重大鼠颈总动脉的钙含量增加、钙盐沉积增多、ALP活性增强,Runx2、Msx2、BMP2、osteocalcin的蛋白和mRNA表达增加,SM22α蛋白和mRNA表达降低(P<0.05或P<0.01),但上述指标在HU4周日与HU8周两组间差异无统计学意义。结论中期和长期模拟失重可引起大鼠颈总动脉平滑肌细胞向成骨样细胞表型转化及动脉钙化,且钙化程度不因模拟失重时间延长发生明显变化。Objective To explore the effects of mid-term (4 weeks) and long-term (8 weeks) simulated microgravity on calcification of the common carotid artery in the rats.Methods Hindlimb-unweighted (HU) rat model was used to simulate the effects of weightlessness on the cardiovascular system.Seventy-five male SD rats were randomized into the control group,4-week HU group (HU4w),and 8-week HU group (HU8w).After the common carotid arteries were separated,the quantification of calcium content and the alizarin red staining were used to detect the calcium salt deposition in the common carotid arteries.The alkaline phosphatase (ALP) activity of common carotid arteries was measured.Western blotting and the real-time polymerase chain reaction (RT-PCR) were performed to measure the protein and mRNA levels of Runx2,Msx2,BMP2,osteocalcin,and SM22α.Results Compared with the control group,the calcium content and the calcium salt deposition were increased,meanwhile,the ALP activity was enhanced in the common carotid arteries of 4-week and 8-week HU rats.Besides,the protein and mRNA levels of Runx2,Msx2,BMP2,osteocalcin were increased,while those of SM22α were decreased in the common carotid arteries of 4-week and 8-week HU rats (P<0.05 or P<0.01).Interestingly,there was no statistically significant difference of above tests between the HU4w and HU8w groups.Conclusion Mid-and long-term simulated microgravity induced the osteogenic transdifferentiation of smooth muscle cells and calcification in the common carotid artery of rats,while the degree of calcification did not change significantly with the prolonged period of simulated microgravity.

关 键 词：模拟失重 大鼠 颈总动脉 血管钙化 血管平滑肌细胞 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]