改良光鸡胚试验评估化妆品用超细二氧化钛的光毒性  

Application of a modified photo hen’s egg test to assess the phototoxicity of ultrafine titanium dioxide particles used in cosmetics

作　　者：刘磊 孔雪 唐颖 LIU Lei;KONG Xue;TANG Ying(Department of Cosmetics,Beijing Technology and Business University,Beijing 100048,China;Beijing Key Laboratory of Plant Research and Development,Beijing 100048,China)

机构地区：[1]北京工商大学化妆品系,北京100048 [2]北京市植物资源研究开发重点实验室,北京100048

出　　处：《日用化学工业》2021年第2期115-120,138,共7页China Surfactant Detergent & Cosmetics

基　　金：国家自然科学基金资助项目(51403006)。

摘　　要：通过对德国人Neumann的光鸡胚试验进行了改良,并对其评估化妆品用超细二氧化钛的光毒性方面进行了初步应用。实验采用2×2阶测试设计,将受试物以无毒浓度作用于培育9 d的鸡胚绒毛尿囊膜(CAM),继而暴露于5 J/cm2的UVA紫外区,去除受试物后再孵育24 h,观察CAM血管反应并计算光刺激指数,区分光毒性效力。通过对10种不同粒径、晶型、表面涂层的超细二氧化钛原料的应用研究发现,该改良光鸡胚试验可作为一种对超细无机防晒原料进行光安全性筛选的简便快捷、高灵敏度的体外替代方法。A modified Neumann’s photo hen’s egg test was developed and applied in the phototoxicity assessment of ultrafine titanium dioxide ingredients used in cosmetics.The test substance was directly applied onto 9-day chicken embryo chorioallantoic membrane(CAM)at non-toxic dosage.After exposure to 5 J/cm2 UVA,the test substance was removed from CAM and eggs were incubated for 24 hours.The vascular responses of CAM and the embryo lethality were observed and a phototoxic index was calculated to quantify the phototoxic potential.Ten ultrafine titanium dioxide samples differing in particle size,crystal form and surface coating were investigated,suggesting that this improved photo-CAM assay could be used as a simple,rapid and sensitive in vitro method in the photosafety screening of ultrafine inorganic sunscreen materials.

关 键 词：鸡胚绒毛尿囊膜 光毒性 动物替代试验 超细二氧化钛 

分 类 号：TQ658[化学工程—精细化工]