诃子水提物体内外抗菌作用及止痒效果研究  

Study of antifungal effect in vivo and in vitro and antipruritic effects of water extract of Terminalia chebula Retz

作　　者：吴小诗 潘敬灵 邓榕榕 莫梓童 唐春萍 沈志滨 WU Xiao-shi;PAN Jing-ling;DENG Rong-rong;MO Zi-tong;TANG Chun-ping;SHEN Zhi-bing(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou,Guangdong 511400 China;Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System,Guangzhou,Guangdong 510006,China;Guangdong cosmetic Engineering Technology Research Center,Guangzhou,Guangdong 510006,China)

机构地区：[1]广东药科大学中药学院,广东广州511400 [2]广东省局部精准药物递药制剂工程技术研究中心,广东广州510006 [3]广东省化妆品工程技术研究中心,广东广州510006

出　　处：《日用化学工业》2021年第2期127-131,共5页China Surfactant Detergent & Cosmetics

基　　金：广东省科技厅应用型研发专项(2015B020234009);国家重点研发计划“中医药现代化”重点专项项目(2018YFC1707100)。

摘　　要：以诃子水提物(TRW)为研究对象,探究其对限制性马拉色菌(Malassezia restricta,M.restricta)体内外抗菌作用及对豚鼠皮肤瘙痒模型的止痒作用。采用试管药基法测定TRW的体外最小抑菌浓度(MIC)。结果显示,对M.restricta的MIC为7.20 mg/mL。采用连续7日涂菌法构建豚鼠皮肤感染M.restricta模型,探讨TRW的体内抗菌活性。结果表明,给药7天后与模型组比较,TRW高、中剂量组可显著降低豚鼠感染皮损评分(P<0.05);TRW低、中、高剂量转阴率分别为37.5%、50%和62.5%;减轻豚鼠病理组织的损伤程度,采用磷酸组胺构建皮肤瘙痒模型探讨TRW的止痒效果。结果显示,TRW明显抑制磷酸组胺所致的瘙痒(P<0.05)。Investigated the effects of Terminalia chebula Retz.(TRW)with water extraction on Malassezia restricta(M.restricta)in vivo and in vitro as well as its antipruritic effects on guinea pig skin pruritus model.The minimal inhibitory concentration(MIC)in vitro of TRW against M.restricta was detected by the test tube dilution method.The results show that the MIC values against M.restricta is 7.20 mg/mL.Experimental cutaneous M.restricta infection was achieved in guinea pigs by daily inoculum for 7 consecutive days,which exproled the antifungal activity of TRW in vivo.The results show that compared with the model group,the high and medium dose of TRW display a reduction of skin lesions score in the guinea pig models(P<0.05)after 7 days of administration,the negative conversion rates of low,middle and high doses of TRW are 37.5%,50%and 62.5%,respectively,which reduce the damage of pathological tissues in guinea pigs.Histamine phosphate inducing skin pruritus in guinea pigs was adopted to observe the effect on pruritus in experimental animals.The results show that TRW obviously inhibites skin pruritus induced by histamine in guinea pigs(P<0.05).

关 键 词：诃子 限制性马拉色菌 真菌感染模型 止痒 

分 类 号：TQ658[化学工程—精细化工]