3种警犬不同肌肉中PPARGC1A基因的发育性表达研究  

Developmental Expression of PPARGC1A Gene in Different Muscles of Three Police Dogs

作　　者：李静 万九生[1,2] 陈超 邓卫东 岳锐[1,2] 熊和丽 黎立光[1,2] LI Jing;WAN Jiu-sheng;CHEN Chao(Kunming Police Dog Base of the Ministry of Public Security,Kunming,Yunnan 650201;Key Laboratory of Police Dog Technology of the Ministry of Public Security,Kunming,Yunnan 650201;School of Animal Science and Technology of Yunnan Agricultural University,Kunming,Yunnan 650201)

机构地区：[1]公安部昆明警犬基地,云南昆明650201 [2]公安部警犬技术重点实验室,云南昆明650201 [3]云南农业大学动物科技学院,云南昆明650201

出　　处：《安徽农业科学》2021年第4期92-95,共4页Journal of Anhui Agricultural Sciences

基　　金：公安部科技强警基础工作专项项目(2018GABJC27,2019GABJC29);云南省科技厅科技计划项目基础研究计划项目(青年项目)(2019FD025)。

摘　　要：[目的]探究PPARGC1A基因mRNA在昆明犬、马里努阿犬、德国牧羊犬不同肌肉组织中的发育性表达规律,为研究犬的肌肉和运动能力形成机理提供理论参考。[方法]采用实时荧光定量PCR技术检测昆明犬、马里努阿犬、德国牧羊犬3种警犬在6月龄、1岁龄的臀腿肌肉组织中PPARGC1A基因mRNA表达量。[结果]随着年龄的增长,PPARGC1A基因在3种警犬肌肉中呈现逐渐上升的表达趋势。马里努阿犬在6月龄和1岁龄时臀腿肌中的表达量极显著高于昆明犬和德国牧羊犬(P<0.01)。[结论]PPARGC1A基因在肌肉发育和肌内脂肪沉积过程中起着很重要的作用。该研究结果可为探索肌肉发育及肌内脂肪沉积相关研究提供理论参考和研究基础。[Objective]The aim of this study was to investigate the development expression laws of PPARGC1A gene mRNA in different muscle tissues of Kunming Dog,Malinua Dog and German Shepherd Dog,and provide theoretical reference for studying the formation mechanism of muscle and motor ability of dogs.[Method]The expression quantity of PPARGC1A gene mRNA in the gluteal and leg muscles of Kunming Dog,Malinua Dog and German Shepherd Dog at 6 month-age and 1-year-age was detected by real-time fluorescence quantitative PCR.[Result]PPARGC1A gene was gradually increased in three kinds of muscles with the increase of age.The expression level of gluteal leg muscle in Malinua Dog was significantly higher than that in Kunming Dog and German Shepherd Dog(P<0.01).[Conclusion]PPARGC1A gene played an important role in muscle development and intramuscular fat deposition.The results of this study could provide theoretical reference and research basis for the study of muscle development and intramuscular fat deposition.

关 键 词：犬 昆明犬 PPARGC1A基因 发育性表达 肌肉组织 

分 类 号：S829.2[农业科学—畜牧学]