八月瓜对四氯化碳所致肝损伤小鼠的保护作用  

Protective Effect of Holboellia latifolia Wall.on Liver Injury Induced by Carbon Tetrachloride in Mice

作　　者：伍沙沙 王延 徐婷 杨燕飞 梁潇 丁晓丽 刘晓龙 钱海兵 WU Sha-sha;WANG Yan;XU Ting(Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550002)

机构地区：[1]贵州中医药大学,贵州贵阳550002

出　　处：《安徽农业科学》2021年第4期163-165,174,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(81760773);贵州省国内一流学科建设项目(GNYL〔2017〕008);贵州省普通高等学校特色重点实验室建设项目(黔教合KY字〔2017〕006)。

摘　　要：[目的]研究八月瓜对四氯化碳(CCl 4)诱导的小鼠急性肝损伤的保护作用。[方法]将60只昆明雄性小鼠随机分为空白对照组、模型组、八月瓜低、中、高剂量组以及护肝片组。每天灌胃给药1次,连续14 d,末次给药1 h后,采用腹腔注射0.1%CCl 4建立小鼠急性肝损伤模型,检测八月瓜对血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(AKP)、γ-谷氨酰转肽酶(γ-GT)和乳酸脱氢酶(LDH)水平的影响;测定肝匀浆中丙二醛(MDA)、还原型谷胱甘肽(GSH)活性;HE染色观察肝脏病理改变。[结果]与模型组比较,八月瓜各剂量组可降低ALT、AST、AKP、γ-GT、LDH和MDA水平,提高GSH活性,改善肝组织病变。[结论]八月瓜能改善CCl 4所致小鼠急性肝损伤,具有一定的保护作用。[Objective]To study the protective effect of Holboellia latifolia on acute liver injury induced by carbon tetrachloride(CCl 4)in mice.[Method]60 Kunming male mice were randomly divided into blank control group,model group,low,medium and high dose of Holboellia latifolia groups and Hugan tablet group.Irrigation to once a day for 14 days,after the last for 1 h,intraperitoneal injection of 0.1%CCl 4 was adopted to establish the acute liver injury in mice model,detection of Holboellia latifolia.on serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(AKP),gamma glutamyl transpeptidase(γ-GT)and lactate dehydrogenase(LDH)level;the activities of malondialdehyde(MDA)and reduced glutathione(GSH)in liver homogenate were measured.HE staining was used to observe the pathological changes of liver.[Result]Compared with the model group,each dose group of Holboellia latifolia could reduce the levels of ALT,AST,AKP,γ-GT,LDH and MDA,increase GSH activity,and improve liver tissue disease.[Conclusion]Holboellia latifolia can improve the acute liver injury induced by CCl 4 in mice,and has a protective effect.

关 键 词：八月瓜 四氯化碳 肝损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]