高温胁迫下不同玉米花粉组蛋白Zm-H3基因的克隆及表达分析  

Cloning and Expression Analysis of Histone Zm-H3 Gene in Different Maize Pollen under High Temperature Stress

作　　者：徐劲松[1] 杨海鹏[1] 张红梅[1,4] 刘小红 郝耀山[3] 孙毅[3] XU Jinsong;YANG Haipeng;ZHANG Hongmei;LIU Xiaohong;HAO Yaoshan;SUN Yi(Institute of Maize,Shanxi Academy of Agricultural Sciences,Xinzhou Shanxi 034000,China;College of Life Sciences,China West Normal University,Nanchong Sichuan 637002,China;Biotechnology Research Center,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China;Key Laboratory of Crop Gene Resources and Germplasm Enhancem Enton Loess Plateau,Ministy of Agriculture,Taiyuan Shanxi 030031,China)

机构地区：[1]山西省农业科学院玉米研究所,山西忻州034000 [2]西华师范大学生命科学学院,四川南充637002 [3]山西省农业科学院生物技术中心,太原030031 [4]农业部黄土高原作物基因资源和种质创制重点实验室,山西太原030031

出　　处：《种子》2021年第1期11-16,共6页Seed

基　　金：山西省农业科学院项目(YGG 17101);四川省南充市研发资金项目(19 YFZJ 0081)共同资助

摘　　要：为了解组蛋白H 3基因(Zm-H3)在不同耐高温玉米(Zea mays L.)材料中的差异表达情况,以中地88(耐高温)和先玉335(不耐高温)2个玉米品种为材料,在高温条件下取其花粉提取总RNA,再通过mRNA纯化、反转录、文库构建及高通量测序等步骤,克隆了一个玉米组蛋白Zm-H3。生物信息学分析表明,该基因位于玉米第6染色体上,全长cDNA为904 bp,编码长度为136个氨基酸的蛋白质,进一步对该蛋白的理化性质、功能位点及结构作了分析。转录组高通量测序结果显示,Zm-H3基因在这2个玉米材料中的表达呈现显著差异,在中地88材料中出现了高表达,为先玉335材料的2.08倍。该实验结果可以为玉米组蛋白H 3基因的应用研究奠定分子基础,也可为其它基因的克隆及表达分析提供参考。To understand differential expression of histone gene Zm-H3 in different high temperature-tolerant maizes,the two maize varieties Zhongdi 88(high temperature-tolerant variety)and Xianyu 335(high temperature-sensitive variety)were used as plant materials to cloned a histone Zm-H3 gene via series of steps including total RNA extract,mRNA purification,reverse transcription,library construction and high-throughput sequencing.Bioinformatics analysis showed that the Zm-H3 gene was located on chromosome 6 of maize with a full-length cDNA of 904 bp and encoded a protein with 136 amino acids.The physical and chemical properties,functional sites and structure of the protein were further analyzed.High-throughput transcriptome sequencing results showed that the expression of Zm-H3 gene was significantly different in the two maizes,with high expression in Zhongdi 88,which was 2.08 folds higher than that in Xianyu 335.The experimental results could lay a molecular foundation for the application of maize histone Zm-H3 gene,and also provided reference for the cloning and expression analysis of other genes.

关 键 词：玉米 组蛋白 转录组 生物信息学 基因表达 

分 类 号：S513.034[农业科学—作物学]