壳聚糖-白蛋白胶束用于构建血液接触材料表面仿生涂层  

Biomimetic coating constructed by chitosan-albumin micelles on the surface of blood contact materials

作　　者：王倩[1] 李莉 王焕然 卞齐豪 翁亚军[2] 陈俊英[2] WANG Qian;LI Li;WANG Huanran;BIAN Qihao;WENG Yajun;CHEN Junying(School of Life Sciences and Engineering, Southweast Jiaotong University, Chengdu 610031, China;School of Materials Sciences and Engineering,Southweast Jiaotong University, Chengdu 610031, China)

机构地区：[1]西南交通大学生命科学与工程学院,成都610031 [2]西南交通大学材料科学与工程学院,成都610031

出　　处：《功能材料》2021年第1期1214-1220,共7页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(31870955,31470921);国家重点研发资助项目(SQ2019YFC110049)。

摘　　要：血管破裂时,血液系统参与凝血反应,起到止血作用。当植入材料与血液接触时会导致凝血级联响应,因此生物材料的血液相容性至关重要。基于纳米材料的独特性能,本研究中,壳聚糖(CS)在交联剂三聚磷酸钠(TPP)的作用下自组装形成CS-TPP胶束,通过静电相互作用在CS-TPP胶束表面接枝牛血清白蛋白(BSA),使其在CS-TPP胶束表面形成一层蛋白膜,研究其血液相容性。通过调整投料比及反应条件,可实现对胶束尺寸的控制。材料学表征结果显示,CS-TPP-BSA胶束的尺寸均一,形态稳定,分散性良好。随后将CS-TPP-BSA胶束通过多巴胺接枝于材料表面,血液试验证实该胶束涂层能够显著提高血液接触材料的血液相容性。该研究为血液接触生物材料的表面改性提供了一种新的手段。As blood system itself is able to start the self-protective coagulation process to stop bleeding immediately in damaged vessel,blood-contacting materials will severely increase the risk of initiating a coagulation cascade.Therefore,it's vital to ensure the good blood compatibility of blood-contacting materials.In this paper,based on the unique properties of nanomaterials,a chitosan micelle was developed(CS-TPP)firstly by cross-linking agent sodium tripolyphosphate(TPP)-assisted self-assembly.Afterwards,bovine serum albumin was grafted on micelle surface via electrostatic interaction to form CS-TPP-BSA micelles for blood compatibility evaluation.The material characterization results showed that CS-TPP-BSA micelles were uniform,stable and well-dispersed.Besides,micelle size could be regulated by optimizing feed ratios and reaction condition.Micelles were then coated on material surface via dopamine,and blood testing indicated that CS-TPP-BSA coating could significantly enhance the blood compatibility,which provided a new surface modification method for blood-contacting materials.

关 键 词：壳聚糖 牛血清白蛋白 自组装 胶束 血液相容性 

分 类 号：R318[医药卫生—生物医学工程]