牛血清白蛋白水热法一步合成氮掺杂碳点荧光检测2,4,6-三硝基甲苯  

One-step Hydrothermal Synthesis of Nitrogen-doped Carbon Dots by Bovine Serum Albumin for Detection of 2,4,6-Trinitrotoluene

作　　者：刘翠娥 黄加玲 王婷婷 王小琴 刘凤娇 李顺兴 LIU Cuie;HUANG Jialing;WANG Tingting;WANG Xiaoqin;LIU Fengjiao;LI Shunxing(College of Chemistry,Chemical Engineering and Environment,Minnan Normal University,Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology,Zhangzhou 363000)

机构地区：[1]闽南师范大学化学化工与环境学院,现代分离分析科学与技术福建省重点实验室,福建漳州363000

出　　处：《分析科学学报》2021年第1期15-21,共7页Journal of Analytical Science

摘　　要：本文以含氮丰富的水溶性蛋白(牛血清白蛋白)为碳源,经水热法一步合成水溶性好、pH稳定性好、耐高离子强度的氮掺杂碳点(N-CDs)。以N-CDs为荧光探针,于B-R缓冲溶液(pH=12)中,与2,4,6-三硝基甲苯(TNT)形成Meisenheimer络合物产生荧光猝灭,TNT浓度在2.6×10^-6~8.0×10^-4 mol/L范围内,与荧光猝灭程度呈现良好的线性关系(R 2=0.9950),检出限为5.1×10^-6 mol/L。In this paper,nitrogen-rich and water-soluble protein(bovine serum albumin)was used as carbon source,and nitrogen-doped carbon dots(N-CDs)with good water solubility,good pH stability,and high ionic tolerance were synthesized in one-step by hydrothermal method.Using N-CDs as a fluorescent probe,Meisenheimer complex was formed after adding TNT which generated fluorescence quenching in B-R buffer(pH=12).The results showed that there was a good linear relationship(R 2=0.9950)between fluorescence quenching and TNT concentration in the range of 2.6×10^-6-8.0×10^-4 mol/L with a detection limit of 5.1×10^-6 mol/L.

关 键 词：牛血清白蛋白 碳点 2 4 6-三硝基甲苯 荧光猝灭 

分 类 号：O657.39[理学—分析化学]