酶联适配体传感器比色检测牛奶中恩诺沙星残留  

Colorimetric Detection of Enrofloxacin Residues in Milk Based on Enzyme-linked Aptasensor

作　　者：赵秋伶 史雅静[1] 张振宇[2] ZHAO Qiuling;SHI Yajing;ZHANG Zhenyu(School of Biomedical and Chemical Engineering,Liaoning Institute of Science and Technology,Benxi 117004;College of Electrical and Information Engineering,Liaoning Institute of Science and Technology,Benxi 117004;College of Mining Industry Technology,Liaoning Technical University,Huludao 125105)

机构地区：[1]辽宁科技学院生物医药与化学工程学院,辽宁本溪117004 [2]辽宁科技学院电气与信息工程学院,辽宁本溪117004 [3]辽宁工程技术大学矿业技术学院,辽宁葫芦岛125105

出　　处：《分析科学学报》2021年第1期51-56,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.21702086);校博士科研启动基金(No.1910B08)。

摘　　要：通过裁剪分析和共聚焦成像鉴定了适配体的结合域,根据结合域位于3′端茎-环的特点,建立了外切核酸酶Ⅰ依赖的酶联核酸适配体比色分析法检测恩诺沙星残留。波长450 nm吸光度与恩诺沙星浓度呈线性关系,方法的线性范围为36~254μg/L,检测限为15μg/L。实际试样检测显示:15个牛奶样本里有6个检出恩诺沙星残留,残留量在43~152μg/L之间。标准加入回收实验的平均回收率在93.5%~99.6%之间,批内变异系数为6.03%~8.12%,批间变异系数为7.31%~13.42%。该方法操作简便、快速,灵敏度高、特异性强,适合批量样品快速分析和现场快速检测。The binding domain of aptamer was investigated through croppping analysis and confocal imaging.Based on the characteristic that the binding domain is located at the 3′end of stem-loop,an exonuclease I-dependent enzyme-linked aptasensor has been developed for colorimetric detection of enrofloxacin residue.The absorbance at 450 nm has a linear relationship with the concentration of enrofloxacin.The limit of detection for the method is 15μg/L and the linear range is 36-254μg/L.The test results of real samples showed that 6 of the 15 milk samples were found to contain enrofloxacin residues and the residual amount was between 43-152μg/L.Standard addition recovery experiments showed that the average recoveries was between 93.5%-99.6%,the intra-batch variation coefficient was from 6.03%to 8.12%,and the inter-batch variation coefficient was from 7.31%to 13.42%.This method has high sensitivity,specificity,simple and fast operation,and is suitable for rapid analysis of batch samples and rapid detection in the field.

关 键 词：恩诺沙星 核酸适配体 外切核酸酶Ⅰ 比色分析 

分 类 号：O657.3[理学—分析化学]