少年足球运动员运动性疲劳后的差异代谢物筛选  

Screening of different metabolites in teenage football players after exercise fatigue

作　　者：郭飞[1] 向茹 王一民[1] 史银斌[1] 曹卫华[1] 池爱平[1] 曹犇[1] GUO Fei;XIANG Ru;WANG Yi-min;SHI Yin-bin;CAO Wei-hua;CHI Ai-ping;CAO Ben(School of Sports,Shaanxi Normal University,Xi’an 710062,China)

机构地区：[1]陕西师范大学体育学院,陕西西安710119

出　　处：《中国应用生理学杂志》2020年第5期465-470,共6页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(31871209);陕西师大青年教师培育项目(QN2018008)。

摘　　要：目的:探索少年足球运动员在运动性疲劳后的差异代谢物变化。方法:以12名男性少年足球运动员(14~16岁)为试验对象,用功率自行车建立包含有氧运动和无氧运动的训练模型:完成6 min 150 W负荷、60~65 r/min的踏骑运动和30 s的负荷按照测试者体重而设定的最大速度踏骑运动,中间休息1 min,重复运动3组,组间休息3 min;测定运动员每组运动后的最大摄氧量值和平均无氧功率值,采集运动训练前后的尿液样本,利用气相-质谱联用(GC-MS)法检测尿液样本,通过数据库筛选潜在的差异代谢物。结果:与运动前相比较,少年足球运动员运动疲劳后的平均无氧功率显著下降(P<0.05),筛选出25个差异代谢物,其中3个代谢物显著升高(P<0.05,0.01),22个代谢物显著降低(P<0.05,0.01);上述差异代谢物的相关代谢通路归属为甘氨酸-丝氨酸-苏氨酸代谢、三羧酸循环、酪氨酸代谢、氮代谢和甘油磷酯代谢通路。结论:少年足球运动员发生运动性疲劳后,机体的代谢物:肌氨酸、L-别苏氨酸、肌酸、丝氨酸、琥珀酸、柠檬酸、4-羟基苯乙酸、羟胺和乙醇胺产生明显的变化,上述差异代谢物可作为少年足球运动员疲劳评价的指标。Objective:To investigate the changes of metabolites of teenage football players after exercise-induced fatigue.Methods:Twelve male teenage football players(14~16 yrs)were selected as experimental subjects in this study.And an exercise model including aerobic and anaerobic exercise as one group exercise was established by using power bicycle:completion 6 min 150 W load,60~65 r/min of riding exercise and 30 s of riding exercise which load was the maximum speed set by the tester's weight.The rest took 1 min in the middle of one group exercise,and repeat 3 times of one group exercise,then rest for 3 min after one group exercise.The maximum oxygen uptake(VO 2 max)and average anaerobic power were measured after each group exercise.Their urine samples were collected before and after the whole exercise model,and gas chromatograph-mass spectrometer(GC-MS)was used to detect the differential metabolites.Results:The teenage football players had a significant decrease in anaerobic capacity after fatigue.Compared with pre-exercise,a total of 25 differential metabolites were screened out,of which 3 metabolites were significantly higher and 22 metabolites were markedly lower.The related metabolic pathways of above differential metabolites were classified as glycine-serine-threonine metabolism,tricarboxylic acid cycle,tyrosine metabolism,nitrogen metabolism and glycerophospholipid metabolism,respectively.Conclusion:After exercise-induced fatigue occurs in teenage football players,the body's metabolites:sarcosine,L-allothreonine,creatine,serine,succinic acid,citric acid,4-hydroxyphenylacetic acid,hydroxylamine,and ethanolamine produce significant changes.The above-mentioned differential metabolites can be used as indicators for teenage football players'exercise-induced fatigue evaluation.

关 键 词：少年 足球运动员 运动性疲劳 尿液 代谢物 代谢通路 

分 类 号：G804.23[文化科学—运动人体科学]