有氧运动对高糖高脂膳食大鼠腓肠肌Nrf2-SOD的影响  

Effects of aerobic exercise on Nrf2-SOD pathway in the gastrocnemius of rats with high-glucose and high-fat diet

作　　者：刘玉倩 张静[3] 高丽娜[3] 王海涛 LIU Yu-qian;ZHANG Jing;GAO Li-na;WANG Hai-tao(School of Sport and Exercise Science,Lingnan Normal University,Zhanjiang 524048;In stitute of Exercise and Health,Lingnan Normal University,Zhanjiang 524048;Department of Physical Education,Hebei Normal University,Shijiazhuang 050024,China)

机构地区：[1]岭南师范学院体育科学学院,广东湛江524048 [2]岭南师范学院运动与健康研究所,广东湛江524048 [3]河北师范大学体育学院,河北石家庄050024

出　　处：《中国应用生理学杂志》2020年第5期481-485,共5页Chinese Journal of Applied Physiology

基　　金：河北省高等学校自然科学重点项目(ZD2017048);岭南师范学院人才专项(ZL2008,ZL2009)。

摘　　要：目的:探讨有氧运动预防大鼠胰岛素抵抗中Nrf2及SOD的变化。方法:24只12月龄SD大鼠随机分为对照组(C)、高糖高脂IR组(IR)和高糖高脂IR并运动组(IRE)。IRE进行递增负荷跑台运动,运动6周。检测腓肠肌T-SOD、CAT、MDA、GSH/GSSG,ELISA法检测腓肠肌8-OHdG含量,Western blot检测腓肠肌Nrf2和GLUT4表达。结果:①IRE组HOMA-IR明显低于IR组(P<0.05);IRE组肌糖原明显高于IR组(P<0.01);②IRE组T-SOD和CAT、GSH/GSSG明显高于IR组(P<0.01);IRE组8-OHdG和MDA明显低于IR组(P<0.01);③IRE组腓肠肌Nrf2和GLUT4明显高于IR组(P<0.01)。结论:有氧运动可激活大鼠腓肠肌Nrf2-SOD通路,提高抗氧化酶活性和糖摄取能力,预防IR发生。Objective:To investigate the changes of nuclear factor erythroid 2-related factor 2(Nrf2)and superoxide dismutase(SOD)in the prevention of insulin resistance in skeletal muscle of rats by aerobic exercise.Methods:Twenty-four 12-month old Sprague-Dawley(SD)rats were randomly divided into control(C),insulin resistance model(IR)and insulin resistance model and exercise(IRE)groups.IR was induced by high-glucose and high-fat diet.Rats of IRE received the increasing load running on the treadmill for 6 W.The activity of total superoxide dismutase(T-SOD),catalase(CAT),Malonaldehyde(MDA)and glutathione/L-glutathione oxidized(GSH/GSSG)were measured by experiment kits.The content of 8-hydroxy-2 deoxyguanosine(8-OHdG)was measured by ELISA Kits.The expressions of Nrf2 and Glucose transporters 4(GLUT4)in gastrocnemius were detected by Western Blot.Results:①Compared with IR,the content of HOMA-IR was decreased in IRE(P<0.05),while the content of muscle glycogen was increased in IRE(P<0.01).②The activities of T-SOD,CAT,and GSH/GSSG in IRE were significantly increased compared with those in IR(P<0.01).Compared with IR,the contents of 8-OHdG and MDA in IRE were significantly decreased(P<0.01).③The expressions of Nrf2 and GLUT4 in gastrocnemius of IRE rats were increased than those in IR(P<0.01).Conclusion:The Nrf2 pathway was activated in gastrocnemius of rats after aerobic exercise,which promoted the activity of antioxidant enzymes,and prevented IR induced by high-glucose and high-fat diet.

关 键 词：核因子E2相关因子2 有氧运动 腓肠肌 胰岛素抵抗 大鼠 

分 类 号：G804.5[文化科学—运动人体科学]