去氢紫堇碱对慢性炎性痛小鼠痛行为学及脊髓炎症因子表达的影响  

Effects of dehydrocorydaline on pain behavior and spinal cord inflammatory factor expression in chronic inflammatory pain mice

作　　者：殷智宇 阮文晴 孙龙 魏盼 宋建钢[1] YIN Zhiyu;RUAN Wenqing;SUN Long;WEI Pan;SONG Jian’gang(Department of Anesthesiology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai201203,China)

机构地区：[1]上海中医药大学附属曙光医院麻醉科,上海201203

出　　处：《中国医药导报》2021年第2期4-7,11,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(81774108);上海中医药大学附属曙光医院“中西医结合研究专项”(SGZXY-201907)。

摘　　要：目的观察去氢紫堇碱(DHC)对慢性炎性痛小鼠的痛觉过敏和脊髓炎症细胞因子表达的影响。方法30只雄性ICR小鼠按随机数字表法分为三组:Sham组、完全弗氏佐剂(CFA)组和CFA+DHC组,每组各10只。Sham组小鼠右后足跖中部皮下注射25μL生理盐水,CFA组和CFA+DHC组小鼠右后足跖中部皮下注射25μL CFA建立模型,建模后1~7 d每天CFA+DHC组小鼠腹腔注射10 mg/kg DHC,CFA组小鼠腹腔注射溶媒,给药后30 min测定小鼠机械缩足反射阈值(PWMT)和热缩足反射潜伏期(PWL);第7天取脊髓腰膨大,Western blot法检测脊髓促炎性细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6和抗炎细胞因子IL-10的蛋白表达。结果整体分析显示,PWMT、PWL的组间比较、时间点比较及交互作用差异均有统计学意义(均P<0.05)。进一步两两比较,组内比较:Sham组PWMT、PWL造模前后比较,差异无统计学意义(P>0.05)。造模后,CFA组、CFA+DHC组PWMT较造模前降低,PWL较造模前缩短,差异均有统计学意义(均P<0.05)。组间比较:造模前,三组PWMT、PWL基础值比较,差异无统计学意义(P>0.05);造模后1~7 d,与CFA组比较,CFA+DHC组PWMT升高,PWL延长,差异均有统计学意义(均P<0.05)。与CFA组比较,CFA+DHC组脊髓促炎性细胞因子TNF-α、IL-1β、IL-6蛋白表达降低,抗炎细胞因子IL-10蛋白表达增加,差异均有统计学意义(P<0.05)。结论DHC可有效缓解CFA诱导的慢性炎性痛小鼠的痛觉过敏,其机制可能与抑制脊髓促炎性细胞因子TNF-α、IL-1β、IL-6的表达和增加抗炎细胞因子IL-10的表达有关。Objective To observe the effects of dehydrocorinine(DHC)on hyperalgesia and spinal cord inflammatory cytokine expression in chronic inflammatory pain mice.Methods According to the random number table method,30 male ICR mice were divided into three groups:Sham group,complete Freund’s adjuvant(CFA)group and CFA+DHC group,with ten mice in each group.Mice in Sham group were subcutaneously injected with 25μL normal saline in the middle of the right hind paw.Mice in CFA group and CFA+DHC group were subcutaneously injected with 25μL CFA into the middle part of right hind paw.From one to seven days after modeling,mice in the CFA+DHC group were intraperitoneally injected with 10 mg/kg DHC every day,and mice in the CFA group were intraperitoneally injected with solvent,and the paw withdrawal mechanical threshold(PWMT)and paw withdrawal latency(PWL)of the mice were determined 30 min after drug administration.On the seventh day,lumbar enlargement was taken,and the protein expressions of the proinflammatory cytokines tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,IL-6 and anti-inflammatory cytokines IL-10 were detected by Western blot.Results Overall analysis showed that there were statistically significant differences in the inter-group comparison,time point comparison and interaction between PWMT and PWL(all P<0.05).Further comparisons were made in pairs.Intra-group comparison:comparison of PWMT and PWL before and after modeling in Sham group showed no statistically significant difference(P>0.05).After modeling,the PWMT of CFA group and CFA+DHC group were lower than those before modeling,and the PWL was shorter than those before modeling,with statistically significant differences(all P<0.05).Comparison between groups:before modeling,the basic values of PWMT and PWL of the three groups showed no statistically significant difference(P>0.05).From one to seven days after modeling,compared with CFA group,PWMT and PWL in CFA+DHC group were increased and prolonged,and the differences were statistically significant(all P<

关 键 词：去氢紫堇碱 炎性痛 痛觉过敏 炎症因子 

分 类 号：R97[医药卫生—药品]