超声波辅助提取对苜蓿总黄酮含量的影响  

Effect of ultrasonic assisted extraction on the content of total flavonoids in Alfalfa

作　　者：韩洲庆 李晨阳 张宇[1,2] 夏永祥 郭新颍 王梦晗 李波[1,2] HAN Zhouqing;LI Chenyang;ZHANG Yu;XIA Yongxiang;GUO Xinying;WANG Menghan;LI Bo(School of Life Sciences,Agriculture and Forestry,Qiqihar University,Qiqihar 161006,China;Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔大学,抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《高师理科学刊》2021年第1期39-42,共4页Journal of Science of Teachers＇College and University

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(135209267,YSTSXK201886);齐齐哈尔大学2019年研究生创新项目(201910232095);齐齐哈尔大学2020年大学生创新创业训练计划项目(202010232062,202010232056,202010232139);齐齐哈尔市科技计划项目(NYGG-201916)。

摘　　要：以龙牧801苜蓿植株干粉为材料,采用超声波辅助提取法,对影响苜蓿总黄酮含量的4个单因素的不同水平进行比较,以分析各因素对苜蓿总黄酮提取量的影响.结果表明,在4个单因素的不同水平下,物料比1∶30(g·mL^-1),乙醇体积分数70%,超声波浸提时间30 min,超声波功率180 W的条件下,苜蓿总黄酮提取量最大,为4.893 mg·g^-1.The dry powder of Longmu 801 Alfalfa plants was used as material.Ultrasonic assisted extraction was used to extract total flavonoids from Alfalfa plants.In order to analyze the effects of various factors on the extraction of total flavonoids from Alfalfa plants.The results showed that,under the conditions of four single factors,material ratio of 1∶30(g·mL^-1),ethanol volume fraction of 70%,ultrasonic extraction time of 30 min and ultrasonic power of 180 W,the maximum extraction amount of Alfalfa flavonoids was obtained,which was 4.893 mg·g^-1.

关 键 词：龙牧801苜蓿 总黄酮 超声波 单因素 

分 类 号：Q94[生物学—植物学]