耐力运动与高脂饮食对Wistar大鼠骨骼肌线粒体融合分裂蛋白表达的影响研究  

Effects of Endurance Exercise and High-Fat Diet on Mitochondrial Dynamics of Skeletal Muscle in Obese Rats

作　　者：郑义 徐晓阳[1] 薄海[2] 张勇[3] ZHENG Yi;XU Xiao-yang;BO Hai;ZHANG Yong(School of Physical Education and Sport Science,South China Normal University,Guangzhou 510000,China;Department of Military Training Medicine,Logistics University of Chinese People's Armed Police Force,Tianjin 300162,China;Tianjin Key Laboratory of Exercise Physiology&Sports Medicine,Tianjin University of Sport,Tianjin 300381,China)

机构地区：[1]华南师范大学体育科学学院,广东广州510000 [2]武警后勤学院军事训练医学教研室,天津300162 [3]天津体育学院天津市运动生理与运动医学重点实验室,天津300381

出　　处：《广州体育学院学报》2021年第1期89-94,共6页Journal of Guangzhou Sport University

基　　金：国家自然科学基金(31771320,31571224,31110103919)。

摘　　要：目的:观察耐力运动与高脂饮食干预对肥胖大鼠骨骼肌线粒体融合与分裂蛋白表达的影响。方法:48只Wistar大鼠分N组(16只)与H组(32只)普食/高脂饲喂造模8周,随后分为NNC组、NNE组、HHC组、HHE组、HNC组与HNE组,NNC组、NNE组、HNC组与HNE组使用普食饲喂9周,HHC组与HHE组继续使用高脂饲喂9周,NNE组、HHE组与HNE组进行9周耐力运动干预。结果:高脂饲喂后大鼠骨骼肌线粒体融合蛋白MFN1、MFN2表达减少,分裂蛋白FIS1表达增加,耐力运动干预增加了高脂饲喂后大鼠骨骼肌线粒体MFN1、MFN2的表达,降低了DRP1、FIS1的表达。结论:高脂饲喂显著降低了骨骼肌线粒体融合蛋白的表达,增加了分裂蛋白的表达,耐力运动干预显著增加了骨骼肌线粒体融合蛋白的表达,显著降低了HFD干预后骨骼肌线粒体分裂蛋白的表达。Objective:To observe the effects of endurance exercise and high-fat diet on mitochondrial fusion and fission protein expression in skeletal muscle of obese rats.Methods:48 Wistar rats were divided into two groups:N group(16)and H group(32),fed normal diet or high fat diet for 8 weeks,and then divided into NNC,NNE,HHC,HHE,HNC and HNE groups respectively.NNC,NNE,HNC,HNE groups were fed normal diet;HHC and HHE groups were constantly fed high fat diet for the last 9 weeks.The NNE,HHE and HNE groups underwent endurance exercise for 9 weeks.Results:After high fat diet feeding,the expressions of MFN1 and MFN2 of mitochondrial fusion proteins in skeletal muscle of rats were decreased,while the expressions of fission proteins FIS1 were increased.The expressions of MFN1 and MFN2 in skeletal muscle of rats after high-fat feeding were increased by endurance exercise intervention,and the expressions of DRP1 and FIS1 were decreased.Conclusion:High-fat diet feeding significantly reduced the expression of mitochondrial fusion protein in skeletal muscle and increased the expression of fission protein.Endurance exercise intervention significantly increased the expression of mitochondrial fusion protein of skeletal muscle,and significantly reduced the expression of fission protein after HFD intervention.

关 键 词：骨骼肌 线粒体 高脂干预 耐力运动 融合 分裂 

分 类 号：G804.2[文化科学—运动人体科学]