载硫化铋及阿霉素靶向相变型纳米粒多模态显像及联合治疗卵巢癌体外实验  

Multi-modal imaging of targeted phase-change nanoparticles containing bismuth sulfide and doxorubicin and combined treatment of ovarian cancer in vitro

作　　者：赵佳雯 张亮[2] 周頔 王志刚[2] 杨心怡 文明[1] ZHAO Jiawen;ZHANG Liang;ZHOU Di;WANG Zhigang;YANG Xinyi;WEN Ming(Department of Radiology,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;Department of Ultrasound,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China)

机构地区：[1]重庆医科大学附属第一医院放射科,重庆400016 [2]重庆医科大学附属第二医院超声科,重庆400010

出　　处：《中国医学影像技术》2021年第1期13-18,共6页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金青年科学基金项目(81801717);重庆医科大学附属第一医院医学科学培育基金(PYJJ2018-15)。

摘　　要：目的制备叶酸靶向载硫化铋(Bi 2 S 3)与阿霉素(DOX)相变型纳米粒(FBS-PFP-PLGA@DOX),观察其基本性能、体外多模态显像及光热联合治疗体外卵巢癌的效果。方法以双乳化法制备FBS-PFP-PLGA@DOX,检测其理化性质;观察其体外多模态显像;采用近红外激光辐照FBS-PFP-PLGA@DOX并实时记录其温度变化;以细胞计数试剂盒(CCK-8)评估光热联合治疗对卵巢癌细胞(SKOV-3)的体外杀伤作用。结果所制备FBS-PFP-PLGA@DOX平均粒径为(255.00±90.82)nm,平均电位为(-7.07±4.33)mV,DOX及Bi 2 S 3包封率分别为(26.77±0.46)%和(87.57±0.12)%。体外实验中,FBS-PFP-PLGA@DOX浓度越高,多模态显像效果越好。激光照射后FBS-PFP-PLGA@DOX迅速升温,并呈浓度依赖性。FBS-PFP-PLGA@DOX纳米粒与SKOV-3细胞孵育并辐照激光后细胞存活率为12.17%,杀伤肿瘤细胞能力高于其余各组。结论所制备FBS-PFP-PLGA@DOX具备体外多模态显像和联合治疗卵巢癌能力。Objective To prepare folate-targeted bismuth sulfide(Bi 2 S 3)and doxorubicin(DOX)phase-change poly(lactic co glycolic acid)(PLGA)nanoparticles(FBS-PFP-PLGA@DOX),and to observe its basic performance,multi-modal imaging and therapeutic effects of photothermal combined with chemotherapy for ovarian cancer in vitro.Methods FBS-PFP-PLGA@DOX were prepared by using double emulsion solvent evaporation method(W/O/W).The basic physicochemical properties and multi-modal imaging effects of FBS-PFP-PLGA@DOX were detected.The temperature variation was recorded in real time by using irradiating FBS-PFP-PLGA@DOX with near-infrared laser.The cell killing effects of photothermal therapy on ovarian cancer cell(SKOV-3)combined with chemotherapy were evaluated with Cell Counting Kit-8(CCK-8).Results The average particle size of prepared FBS-PFP-PLGA@DOX was(255.00±90.82)nm,and the average zeta potential of FBS-PFP-PLGA@DOX nanoparticles was(-7.07±4.33)mV.The entrapment efficiency of DOX and Bi 2 S 3 was(26.77±0.46)%and(87.57±0.12)%,respectively.The multi-modal imaging effects gradually enhanced with the increasing concentration of [e-mail] near-infrared laser irradiation,the nanoparticles were rapidly warmed up in a concentration-dependent manner.The cell survival rate of FBS-PFP-PLGA@DOX after laser irradiation was 12.17%,killing tumor cells better than the other groups.Conclusion The prepared FBS-PFP-PLGA@DOX had the ability of multi-modal imaging and combined treatment of ovarian cancer in vitro.

关 键 词：硫化铋 阿霉素 光化学疗法 多模态成像 

分 类 号：R-331[医药卫生—影像医学与核医学] R445[医药卫生—诊断学]