两株1型鸭甲肝病毒山东分离株的鉴定与致病性分析  

Identification and Pathogenicity of Two Duck Hepatitis A Virus Type 1 Isolated in Shandong,China

作　　者：王宇飞 孙伟 陈佶慧 刘芹防[2] 滕巧泱[2] 杨健美[2] 苑纯秀[2] 李泽君[2] 李雪松[2,3] 关平原 WANG Yufei;SUN Wei;CHEN Jihui;LIU Qinfang;TENG Qiaoyang;YANG Jianmei;YUAN Chunxiu;LI Zejun;LI Xuesong;GUAN Pingyuan(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018 [2]中国农业科学院上海兽医研究所,上海200241 [3]中国农业大学动物医学院,北京100193

出　　处：《中国动物传染病学报》2021年第1期26-30,共5页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金项目(31702237,31771000);上海市自然科学基金项目(17ZR1437400);国家重点研发计划“家禽重要疫病诊断与检测新技术研究”(2016YFD0500800);上海科委创新行动计划农业项目(17391901700)。

摘　　要：鸭病毒性肝炎(DVH)是危害雏鸭的一种急性高度致死性传染病,引发DVH的主要病原体为鸭甲肝病毒(DHAV),其中1型鸭肝炎病毒在中国各省流行最广。本研究于2018年从山东某养鸭场的病料中分离得到两株DHAV,经分子生物学鉴定、序列比对和进化树分析,鉴定引起雏鸭患病的病原体为DHAV-1。经过纯化后,对SPF雏鸭进行致病性试验,用鸭胚滴定和荧光定量的方法测定脏器病毒含量,致病性分析结果显示,两株DHAV-1对雏鸭的致病性不同,SD37毒株引起雏鸭致死率为100%,而SD63引起雏鸭致死率为12.5%,这可能与VP1氨基酸差异有关。本研究通过对山东地区两株DHAV-1的鉴定和致病性研究,为我国DHAV的分子流行病学、防治以及疫苗研制提供了材料和理论指导。Duck viral hepatitis(DVH)is a highly contagious and fatal disease in ducklings.Duck hepatitis A virus(DHAV)is a major etiological agent and DHAV-1 is widely prevalent in many provinces of China.Two strains of DHAV-1 were isolated in 2018 from a sick duck farm in Shandong province and characterized for their molecular biology,sequences,phylogenetic tree and pathogenicity.The isolated two strains were inoculated into duck embryos and virus titers were determined with a fluorescence assay.Then,SPF ducklings were infected with these two strains and pathological changes were observed in tissues.As a result,SD37 strain caused 100%deaths and SD63 strain had 12.5%mortality.The results of the present study provided a basis for future research on molecular epidemiology and control of DHAV-1 in China.

关 键 词：鸭肝炎病毒 DHAV-1 鉴定 致病性 

分 类 号：S852.659.6[农业科学—基础兽医学]