Microvesicles(MIVs)secreted from adipose-derived stem cells(ADSCs)contain multiple microRNAs and promote the migration and invasion of endothelial cells  

作　　者：Bo Huang Lin-Feng Huang Ling Zhao Zongyue Zeng Xi Wang Daigui Cao Lijuan Yang Zhenyu Ye Xian Chen Bin Liu Tong-Chuan He Xiaozhong Wang 

机构地区：[1]Ministry of Education Key Laboratory of Diagnostic Medicine and School of Laboratory Medicine,and The Affiliated Hospitals of Chongqing Medical University,Chongqing Medical University,Chongqing,400016,China [2]Molecular Oncology Laboratory,Department of Orthopaedic Surgery and Rehabilitation Medicine,The University of Chicago Medical Center,Chicago,IL,60637,USA [3]Department of Clinical Laboratory Medicine,The Second Affiliated Hospital of Nanchang University,Nanchang,330006,China [4]Department of Clinical Laboratory Medicine,Jiangxi Maternal and Child Health Hospital,Nanchang,330006,China [5]Department of Orthopaedic Surgery,Chongqing General Hospital Affiliated with the University of Chinese Academy of Sciences,Chongqing,400013,China [6]Department of Obstetrics and Gynecology,The First Hospital of Lanzhou University,Lanzhou,730030,China [7]Department of General Surgery,The Second Affiliated Hospital of Soochow University,Suzhou,215004,China [8]Department of Clinical Laboratory Medicine,The Affiliated Hospital of Qingdao University,Qingdao,266061,China [9]Department of Biology,School of Life Sciences,Southwest University,Chongqing,400715,China

出　　处：《Genes & Diseases》2020年第2期225-234,共10页基因与疾病（英文）

基　　金：The reported work was supported in part by research grants from the Natural Science Foundation of Jiangxi Province China(#20151BAB215005);the Natural Science Foundation of China(#81660029,81360083);TCH was also supported by the Mabel Green Myers Research Endowment Fund,USA and The University of Chicago Orthopaedics Alumni Fund,USA.Funding sources were not involved in the study design;in the collection,analysis and interpretation of data,in the writing of the report;and in the decision to submit the paper for publication.

摘　　要：Extracellular vesicles(EVs)such as microvesicles(MIVs)play an important role in intercellular communications.MIVs are small membrane vesicles sized 100e1000 nm in diameter that are released by many types of cells,such as mesenchymal stem cells(MSCs),tumor cells and adipose-derived stem cells(ADSC).As EVs can carry out autocrine and paracrine functions by controlling multiple cell processes,it is conceivable that EVs can be used as delivery vehicles for treating several clinical conditions,such as to improve cardiac angiogenesis after myocardial infarction(MI).Here,we seek to investigate whether ADSC-derived MIVs contain microRNAs that regulate angiogenesis and affect cell migration of endothelial cells.We first characterized the ADSC-derived MIVs and found that the MIVs had a size range of 100 e300 nm,and expressed the MIV marker protein Alix.We then analyzed the microRNAs in ADSCs and ADSC-derived MIVs and demonstrated that ADSC-derived MIVs selectively released a panel of microRNAs,several of which were related to angiogenesis,including two members of the let-7 family.Furthermore,we demonstrated that ADSC-derived MIVs promoted the cell migration and invasion of the HUVEC endothelial cells.The PKH26-labeled ADSC-derived MIVs were effectively uptaken into the cytoplasm of HUVEC cells.Collectively,our results demonstrate that the ADSC-derived MIVs can promote migration and invasion abilities of endothelial cells,suggesting pro-angiogenetic potential.Future studies should focus on investigating the roles and mechanisms through which ADSC-derived MIVs regulate angiogenesis.

关 键 词：Adipose-derived stem cells(ADSCs) Angiogenesis Let-7 microRNA Microvesicle(MIV) 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]