榆栀止血颗粒含药血清对异位子宫内膜间质细胞PTEN/PI3K/AKT信号通路的影响  

Effect of Serum Containing Yuzhi Zhixue Granule on PTEN/PI3K/AKT Signal Pathway in Ectopic Endometrial Stromal Cells

作　　者：刘艳杰[1] 杨阳[2] LIU Yanjie(The First Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450000,China)

机构地区：[1]郑州大学第一附属医院妇科,河南郑州450000 [2]南京医科大学附属南京医院妇科,江苏南京210006

出　　处：《河北医学》2021年第2期177-181,共5页Hebei Medicine

基　　金：国家重大新药创制专项榆栀止血颗粒的临床研究,(编号:2015ZX09107-001)。

摘　　要：目的:探讨榆栀止血颗粒含药血清对异位子宫内膜间质细胞(ESCs)中第10号染色体同源丢失性磷酸酶张力蛋白(PTEN)/磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKT)信号通路的影响。方法:制备榆栀止血颗粒含药血清,体外培养人异位ESCs细胞,分为对照组(空白血清)、低剂量组(榆栀止血颗粒低剂量血清)、中剂量组(榆栀止血颗粒中剂量血清)和高剂量组(榆栀止血颗粒高剂量血清)。CCK-8法检测细胞增殖情况;Transwell法检测细胞侵袭情况;实时荧光定量PCR(qRT-PCR)法检测细胞中PTEN mRNA表达情况;蛋白印迹(WB)法检测细胞中PTEN、p-PI3K、PI3K、p-AKT、AKT蛋白表达情况。结果:与对照组相比,低、中、高剂量组异位ESCs细胞OD值、侵袭细胞数、p-PI3K/PI3K、p-AKT/AKT蛋白表达水平显著降低(P<0.05),细胞中PTEN mRNA及蛋白表达水平显著升高(P<0.05);随着榆栀止血颗粒含药血清剂量升高,异位ESCs细胞OD值、侵袭细胞数、p-PI3K/PI3K、p-AKT/AKT蛋白表达水平逐步递减(P<0.05),细胞中PTEN mRNA及蛋白表达水平逐步递增(P<0.05)。结论:榆栀止血颗粒含药血清可抑制异位ESCs细胞增殖与侵袭,上调PTEN表达并抑制PI3K/AKT信号通路。Objective:To investigate the effect of serum containing Yuzhi Zhixue granule on the signal pathway of phosphatase and tensin homolog deleted on chromosome ten(PTEN)/phosphatidyl inositol-3-kinase(PI3K)/protein kinase B(AKT)in ectopic endometrial stromal cells(ESCs).Methods:The serum containing Yuzhi Zhixue granule was prepared,and human ectopic ESCs cells were cultured in vitro,which were divided into control group(blank serum),low dose group(low dose Yuzhi Zhixue granules serum),middle dose group(middle dose Yuzhi Zhixue granules serum)and high dose group(high dose Yuzhi Zhixue granules serum).CCK-8 method was used to detect cell proliferation;cell invasion was detected by Transwell method;the expression of PTEN mRNA was detected by real-time fluorescent quantitative PCR(qRT-PCR);and the expressions of PTEN,p-PI3K,PI3K,p-AKT and AKT proteins were detected by Western blot(WB).Results:Compared with those in the control group,the OD value,invasive cell number,p-PI3K/PI3K,p-AKT/AKT proteins expression levels of ectopic ESCs cells in the low,middle and high dose groups were significantly lower(P<0.05),and the expressions of PTEN mRNA and protein were significantly higher(P<0.05);at the same time,with the increase of dosage of serum containing Yuzhi Zhixue granule,the OD value,invasive cell number,p-PI3K/PI3K,p-AKT/AKT protein expression level of ectopic ESCs cells decreased gradually(P<0.05),and the expressions of PTEN mRNA and protein increased gradually(P<0.05).Conclusion:Serum containing Yuzhi Zhixue granule can inhibit the proliferation and invasion of ectopic ESCs cells,up-regulate the PTEN expression and inhibit PI3K/AKT signaling pathway.

关 键 词：榆栀止血颗粒含药血清 异位子宫内膜间质细胞 第10号染色体同源丢失性磷酸酶张力蛋白/磷脂酰肌醇3-激酶/蛋白激酶B 

分 类 号：R285[医药卫生—中药学]