伐地那非对豚鼠药物性聋听功能及耳蜗毛细胞形态学的影响  

Effects of Vardenafil on the Hearing Function and Cochlea Morphology of Guinea Pigs with Drug Induced Hearing Loss

作　　者：梁媛[1] 黄跃雁[1] 郭霞 刘淑红[1] 刘吉娜[1] 曹静 LIANG Yuan;HUANG Yueyan;GUO Xia(The Affiliated Hospital of Chengde Medical College,Hebei Chengde 067000,China)

机构地区：[1]承德医学院附属医院耳鼻喉科,河北承德067000

出　　处：《河北医学》2021年第2期186-190,共5页Hebei Medicine

基　　金：河北省承德市科学技术研究与发展计划项目,(编号:201801A048)。

摘　　要：目的:探讨伐地那非(vardenafil)对豚鼠药物性聋听功能及耳蜗形态学的影响。方法:入组的30只健康豚鼠运用数字表法随机分成模型对照组10只、治疗组10只以及空白对照组10只。其中模型对照组和治疗组在连续腹腔注射庆大霉素120mg·kg^-1·d^-12周后分别腹腔注射NaCl注射液4mL·kg^-1·d^-1及伐地那非1mg·kg^-1·d^-1,连续给药4周。分别测试造模前1d、造模后2周、给药1、2及4周后听性脑干反应(ABR)阈值,并通过扫描电镜观察耳蜗毛细胞的形态变化。结果:在庆大霉素给药2周后,治疗组及模型对照组ABR阈值均较空白对照组有明显升高,差异具有统计学意义(P值均<0.01)。在注射伐地那非后治疗组ABR阈移小于模型对照组,差异具有统计学意义(P值均<0.01)。扫描电镜显示,模型对照组豚鼠耳蜗外毛细胞出现听毛紊乱、融合及缺失;而治疗组耳蜗病变较轻,听毛仅有轻微倒伏、融合现象。结论:伐地那非能够有效降低庆大霉素所导致的豚鼠耳蜗毛细胞损害程度,显示出其对药物性听力损伤具有明显的改善作用。Objective:To study the effects of vardenafil on the hearing function and cochlea morphology of guinea pigs with drug induced deafness.Methods:Guinea pigs were randomly divided into blank control group,model control group and treatment group,10 in each group.Two weeks after gentamicin 120mg·kg^-1·d^-1 injected into Guinea pigs of treatment group and model control group,vardenafil(1mg·kg^-1·d^-1)and NS(4ml·kg^-1·d^-1)were injected into Guinea pigs of the above two groups respectively.ABR thresholds were measured respectively prior to the experiment,1,2 and 4 weeks post-drugs.The morphological changes of cochlea hair cells were observed by scanning electron microscope.Results:After 2 weeks of gentamicin administration,the ABR threshold of treatment group and model control group were significantly higher than that of the blank control group,with statistically significant differences(all P values<0.01).After vardenafil injection,the ABR threshold shift in the treatment group was smaller than that in the model control group,and the difference was statistically significant(all P<0.01).SEM showed that hair cells of the inner and outer hair cells in model control group displayed mess,fusion and imperfections.In the treatment group,the hair cells displayed slight pathological changes,there wasn't significant difference compared with blank control group.Conclusion:Vardenafil can effectively reduce the damage degree of guinea pig cochlea hair cells caused by gentamicin,which shows that it has obvious improvement effect on drug-induced hearing impairment.

关 键 词：伐地那非 药物性聋 耳蜗 形态学 

分 类 号：R764[医药卫生—耳鼻咽喉科]