HIV母婴阻断新生儿早期肠道菌群特征分析  

Structural features of intestinal flora in early stage of neonates who had received intervention for preventing mother-to-child transmission of HIV

作　　者：马丙南 朱炜春[1] 林菁[1] 周洁雯 高智群[1] MA Bingnan;ZHU Weichun;LIN Jing;ZHOU Jiewen;GAO Zhiqun(Guangzhou Eighth People's Hospital,Guangzhou 510440,China)

机构地区：[1]广州市第八人民医院,广州510440

出　　处：《山东医药》2021年第5期31-35,共5页Shandong Medical Journal

基　　金：广东省医学科学技术研究基金项目(B2018166);广东省中医药局科研项目(20202145)。

摘　　要：目的分析人类免疫缺陷病毒(HIV)母婴阻断新生儿早期肠道菌群特征,寻找影响HIV母婴阻断新生儿神经功能发育的潜在原因。方法选取HIV母婴阻断新生儿20例(母婴阻断组)及同期与之相匹配的健康母亲所生新生儿20例(对照组),均在新生儿出生后第7天留取粪便标本;先行肠道菌群DNA提取和纯化,应用Illumina Hiseq 2500测序平台进行细菌16 S高通量测序,分析肠道菌群alpha多样性(包括Observed Species指数、Chao1指数及Shannon指数),并比较肠道优势菌群在门、科、属水平的相对丰度。结果母婴阻断组Observed Species指数及Chao1指数均低于对照组(P均<0.05),两组Shannon指数比较差异无统计学意义。母婴阻断组拟杆菌门、双歧杆菌科、双歧杆菌属及梭菌属相对丰度低于对照组(P均<0.05),两组其他菌在门、科、属水平的相对丰度比较差异无统计学意义。结论HIV母婴阻断新生儿早期的肠道菌群多样性降低,拟杆菌门、双歧杆菌科、双歧杆菌属及梭菌属相对丰度降低;这些差异可能导致肠道菌群产生的代谢产物或神经递质异常,对HIV母婴阻断新生儿神经发育过程产生不利影响。Objective To analyse the structural features of intestinal flora in early stage of neonates who had re⁃ceived intervention for prevention of mother-to-child transmission(PMTCT)of human immunodeficiency virus(HIV)and to explore its potential impact on neurodevelopment.Methods The neonates who had received intervention for PMTCT were enrolled into the PMTCT group(20 cases).The neonates who were born by healthy mothers were enrolled into the control group(20 cases).Fecal samples were collected on the 7th day after birth in two groups.Total microbial genomic deoxyribonucleic acid(DNA)was extracted and purified from the fecal samples.The bacterial 16S ribosomal deoxyribonucleic acid(rDNA)genes were selectively amplified and a 16S rDNA clone library was constructed by using Illumina hiseq 2500 sequencing platform.The alpha diversity information such as observed species index,Chao1 index,and Shannon index of intestinal flora was analyzed,and the relative abundance of dominant intestinal flora in phylum,family,and genus were compared between these two groups.Results The observed species index and Chao1 index of intestinal flora were lower in the PMTCT group than in the control group(all P<0.05).There was no significant difference in Shannon index between the two groups.The relative abundances of Bacteroides,Bifidobacteriaceae,Bifidobacterium,and Clostridium in the PMTCT group were lower than those of the control group(all P<0.05).There were no significant differences in relative abundance of other dominant bacteria in phylum,family and genus between the two groups.Conclusions The diversity of intestinal flora in early stage of neonates who had received intervention for PMTCT is lower than that of the normal neonates,and the relative abundances of Bacteroides,Bifidobacteriaceae,Bifidobacterium and Clostridium are lower.These differences in intestinal flora may lead to abnormal metabolites or neurotransmitters,which might have adverse effects on neonatal neurodevelopment.

关 键 词：人类免疫缺陷病毒 母婴阻断 肠道菌群 菌群多样性 神经发育 新生儿 

分 类 号：R722.1[医药卫生—儿科]