舒芬太尼调控LINC00668对卵巢癌细胞增殖与凋亡的影响  

Effects of sufentanil on the proliferation and apoptosis of ovarian cancer cells regulated by LINC00668

作　　者：王亚娟[1] 宫伟 黄珊 WANG Yajuan;GONG Wei;HUANG Shan(Department of Anesthesiology, Baoding Maternal and Child Health Hospital, Baoding 071000, Hebei, China;Department of Anesthesiology, Baoding No.1 Hospital, Baoding 071000, Hebei, China)

机构地区：[1]保定市妇幼保健院麻醉科,河北保定071000 [2]保定市第一医院麻醉科,河北保定071000

出　　处：《西部医学》2021年第2期205-210,共6页Medical Journal of West China

基　　金：保定市科技计划项目(1941ZF097)。

摘　　要：目的研究舒芬太尼调控LINC00668对卵巢癌细胞增殖、凋亡的影响。方法分别给予终浓度为25 ng/mL、50 ng/mL、100 ng/mL的舒芬太尼处理A2780细胞,记为实验1、2、3组,正常培养的细胞作为对照组;将si-NC、si-LINC00668分别转染至A2780细胞中,记为si-NC组、si-LINC00668组;将pcDNA3.1、pcDNA3.1-LINC00668转染至A2780细胞中,同时用100 ng/mL的舒芬太尼处理,记为实验3组+pcDNA3.1组、实验3组+pcDNA3.1-LINC00668组。流式细胞仪检测细胞周期;四甲基偶氮唑盐比色法(MTT)检测细胞存活率;蛋白质印迹(Western blot)法检测Ki67、pro-caspase-3、clv-caspase-3蛋白表达;实时荧光定量PCR(RT-qPCR)检测LINC00668表达水平;流式细胞术检测细胞凋亡。结果50 ng/mL、100 ng/mL舒芬太尼处理的A2780细胞中G0期细胞所占比例显著升高,S期细胞所占比例显著降低(P<0.05),G1期细胞所占比例无显著变化(P>0.05);细胞存活率显著降低,细胞凋亡率显著升高,Ki67、pro-caspase-3表达水平显著降低,clv-caspase-3表达水平显著升高,LINC00668表达水平显著降低(P<0.05)。抑制LINC00668表达,G0期细胞所占比例显著升高,S期细胞所占比例显著降低(P<0.05),G2期细胞所占比例无显著变化(P>0.05);细胞存活率显著降低,细胞凋亡率显著升高,Ki67、pro-caspase-3表达水平显著降低,clv-caspase-3表达水平显著升高(P<0.05)。过表达LINC00668逆转了舒芬太尼对A2780增殖、凋亡的影响。结论舒芬太尼可能通过下调LINC00668的表达抑制卵巢癌细胞增殖,促进细胞凋亡。Objective To study the effect of sufentanil on the proliferation and apoptosis of ovarian cancer cells.Methods A2780 cells treated with sufentanil at final concentrations of 25 ng/mL,50 ng/mL,and 100 ng/mL were recorded as groups 1,2,and 3 of the experiment.Normally cultured cells were used as the control group.si-NC,si-LINC00668 was transfected into A2780 cells and recorded as si-NC group and si-LINC00668 group.pcDNA3.1 and pcDNA3.1-LINC00668 were transfected into A2780 cells and treated with 100 ng/mL sufentanil,and recorded as the experimental 3 group+pcDNA3.1 group,the experimental 3 group+pcDNA3.1-LINC00668 group.Flow cytometry was used to detect the cell cycle.Tetramethylazozolium colorimetry(MTT)was used to detect cell survival rate.Western blot was used to detect Ki67,pro-caspase-3,and clv-caspase-3 protein expression.Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect the expression of LINC00668.Flow cytometry was used to detect apoptosis.ResultsThe proportion of G0 phase cells in A2780 cells treated with 50 ng/mL and 100 ng/mL sufentanil was significantly increased.The proportion of S phase cells was decreased significantly(P<0.05).The proportion of G1 phase cells was not change significantly(P>0.05).The cell survival rate was significantly reduced.The apoptotic rate was significantly increased.Ki67 and pro-caspase-3 expression levels were significantly reduced.clv-caspase-3 expression was significantly increased,and LINC00668 expression was significantly reduced(P<0.05).The proportion of cells in the G0 phase was increased significantly.The proportion of cells in the S phase was decreased significantly(P<0.05),and the proportion of cells in the G2 phase was not change significantly(P>0.05).The cell survival rate was decreased significantly,and apoptosis rate was significantly increased,Ki67 and pro-caspase-3 expressions were significantly reduced,and clv-caspase-3 expression was significantly increased(P<0.05).Overexpression of LINC00668 reversed the effects of sufentanil on A2780 prolif

关 键 词：舒芬太尼 LINC00668 卵巢癌 增殖 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]