高通量测序分析蚕豆种子内生细菌的多样性  

Diversity of Endophytic Bacteria in Faba Bean Seeds by High-Throughput Sequencing

作　　者：刘璐 名晓东 张晓艳[2] 郝俊杰[2] 付丽平 王乾坤 吕鑫 陈旺 刘全兰 LIU Lu;MING Xiaodong;ZHANG Xiaoyan;HAO Junjie;FU Liping;WANG Qiankun;LYU Xin;CHEN Wang;LIU Quanlan(College of Marine Science and Biological Engineering,Qingdao University of Science&Technology,Shandong Qingdao 266042,China;Qingdao Academy of Agricultural Sciences,Shandong Qingdao 266100,China)

机构地区：[1]青岛科技大学海洋科学与生物工程学院,山东青岛266042 [2]青岛市农业科学研究院,山东青岛266100

出　　处：《中国农业科技导报》2021年第2期73-80,共8页Journal of Agricultural Science and Technology

基　　金：国家食用豆产业技术体系项目(CARS-08);青岛市农业科学研究院院长基金项目。

摘　　要：为了分析蚕豆种子内生细菌的多样性,采用Illumina MiSeq高通量测序技术,从日本大白皮(S18P23.1、S18P23.2)和启豆2号(S18P24.1、S18P24.2)的种子获得16S RNA V3~V4区有效序列133855条。将高于97%相似度的序列划分为一个操作分类单元(operational taxonomic unit,OTU),优化后得到1598个OTUs。内生细菌种群分析结果表明,拟杆菌门(Bacteroidetes,丰度为30%~33%)、变形菌门(Proteobacteria,23%~25%)、厚壁菌门(Firmicutes,23%~25%)和放线菌门(Actinobacteria,5%~7%)为两个蚕豆品种共有的优势菌门,但属水平的优势菌群在供试蚕豆种子中均有差异。这些结果表明,蚕豆种子具有丰富的内生细菌资源,含有多种具有益功能性状的细菌类群,值得进一步跟踪研究这些益生菌在蚕豆种植到土壤后的变化规律;启豆2号种子中益生菌的种类和丰度高于日本大白皮,值得进一步分离和研究这些益生菌的益生或促生特性,筛选获得在食品和生物肥料等领域有应用潜力的菌株。In order to analyze the species abundance and diversity of endophytic bacteria in faba bean seeds,taking Japan’s white skin(S18 P23.1,S18 P23.2)and Qidou 2(S18 P24.1,S18 P24.2)as materials,133855 effective sequences were obtained by Illumina MiSeq high-throughput sequencing of the 16 S rRNA V3~V4 region.The sequence with similarity above 97%was divided into one operational taxonomy unit(OTU),so 1598 OTUs were obtained after optimization.The results showed that majority of the endophytic bacteria belonged to Bacteroidetes(30%~33%),Proteobacteria(23%~25%),Firmicutes(23%~25%),and Actinobacteria(5%~7%),which were therefore the dominant bacterial Phyla in these four faba bean seeds.The dominant genera at genus level in these endophytic bacteria sequenced from faba bean seeds were different.Above results indicated faba bean seeds were rich in endophytic bacteria,the abundance of probiotics in one faba bean variety(Qidou 2)were higher than another variety(Japan’s white skin).

关 键 词：高通量测序 蚕豆 内生细菌 多样性 

分 类 号：S664.143.6[农业科学—果树学]