绝经后妇女不同中医证型膝骨关节炎关节软骨ERRα/SOX9信号通路表达分析  

Analysis of ERR a/SOX9 Signaling Pathway in Articular Cartilage of Postmenopausal with Different Traditional Chinese Medicine Syndromes of Knee Osteoarthritis

作　　者：李朋 李钊 罗天 胡仕畅 霍少川[3] LI Peng;LI Zhao;LUO Tian;HU Shichang;HUO Shaochuan(Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510240,China;Guangzhou University of Traditional Chinese Medicine,Guangzhou 510450,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学第三附属医院,广州510240 [2]广州中医药大学,广州510405 [3]广州中医药大学第一附属医院,广州510405

出　　处：《世界中医药》2021年第2期329-334,共6页World Chinese Medicine

基　　金：广东省中医药局科研项目(20181140)。

摘　　要：目的:探讨ERRα/SOX9信号通路在绝经后妇女不同证型膝骨关节炎(KOA)关节软骨中的作用。方法:选取2018年6月至2019年4月广州中医药大学第三附属医院收治的绝经后KOA患者,根据中医证型标准分为风寒湿痹证、风湿热痹证、瘀血闭阻证和肝肾亏虚证4组,采用RT-PCR和Western Blot检测ERRα/SOX9信号通路表达情况。结果:各组间膝关节软骨组织ERRα、SOX9和Col2α1表达量比较差异有统计学意义(P<0.05)。与风寒湿痹组比较,风湿热痹组ERRα、SOX9和Col2α1表达量之间差异无统计学意义(P>0.05);瘀血痹阻组ERRα表达量升高,SOX9、Col2α1表达量降低(P<0.05);肝肾亏虚组ERRα、Col2α1表达量降低(P<0.05),SOX9表达量升高(P<0.05)。与风湿热痹组比较,瘀血痹阻组ERRα表达量升高,SOX9、Col2α1表达量降低(P<0.05);肝肾亏虚组ERRα、Col2α1表达量降低(P<0.05),SOX9表达量升高(P<0.05)。与瘀血痹阻组比较,肝肾亏虚组ERRα、Col2α1表达量降低(P<0.05);SOX9表达量升高(P<0.05)。结论:绝经后妇女不同中医证型KOA患者关节软骨组织ERRα/SOX9信号通路存在差异,揭示不同证型ERRα/SOX9信号通路的差异将为中医药辨证施治绝经后KOA提供理论基础。Objective:To investigate the role of ERRa/SOX9 signaling pathway in postmenopausal women with different types of knee osteoarthritis articular cartilage.Methods:Postmenopausal KOA patients admitted to Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine were selected as the research objects and were divided into 4 groups according to TCM syndrome type criteria:wind-cold-dampness arthralgia syndrome,wind-dampness-heat arthralgia,blood stasis arthralgia syndrome,and liver-kidney deficiency syndrome.Rt-pcr and Western Blot were used to detect the ERR alpha/SOX9 signal pathway expression.Results:The difference of ERR-alpha,SOX9 and Col2-alpha-1 expression was statistically significant(P<0.05).Compared with wind-cold-dampness arthralgia group,there was no statistically significant difference in the expression of ERRα,SOX9 and Col2α1 in wind-dampness-heat arthralgia groups(P<0.05).Compared with the blood stasis arthralgia瘀血痹阻group,there was no significant difference in the expression of ERRα,SOX9 and Col2α1 in the wind-dampness-heat arthralgia group(P>0.05);the expression of ERRαincreased in the blood stasis arthralgia group,and the expression of SOX9 and Col2α1 decreased(P<0.05);the expression of ERRαand Col2α1 in the liver and kidney deficiency group decreased(P<0.05),and the expression of SOX9 increased(P<0.05).Compared with the wind-dampness-heat arthralgia,the expression of ERRαin the blood stasis arthralgia increased,and the expression of SOX9 and Col2α1 decreased(P<0.05);the expression of ERRαand Col2α1 in the liver and kidney deficiency group decreased(P<0.05),and the expression of SOX9 increased(P<0.05).Compared with blood stasis arthralgia group,the expression of ERRαand Col2α1 in the liver and kidney deficiency group decreased(P<0.05);the expression of SOX9 increased(P<0.05).Conclusion:There are differences in the ERR alpha/SOX9 signaling pathways in the articular cartilage of postmenopausal women with different TCM syndromes of KOA.The discovery of the diff

关 键 词：膝骨关节炎 ERRα/SOX9信号通路 绝经后妇女 关节软骨 中医辨证分型 

分 类 号：R271.9[医药卫生—中西医结合] R274.9[医药卫生—中医妇科学]