绿豆胰蛋白酶抑制剂对绿豆象生长发育及体内解毒酶和保护酶活性的影响  

Effects of mung bean trypsin inhibitor on the growth and development and the activities of detoxifying and protective enzymes in Callosobruchus chinensis(Coleoptera:Bruchidae)

作　　者：樊艳平 党海燕 王宏民 郑海霞[4] 成小芳 张耀文[6] 张仙红[4] FAN Yan-Ping;DANG Hai-Yan;WANG Hong-Min;ZHENG Hai-Xia;CHENG Xiao-Fang;ZHANG Yao-Wen;ZHANG Xian-Hong(College of Geography,Taiyuan Normal University,Jinzhong,Shanxi 030619,China;Taiyuan Customs,People’s Republic of China,Taiyuan 030006,China;College of Economics and Management,Shanxi Agricultural University,Taigu,Shanxi 030801,China;College of Plant Protection,Shanxi Agricultural University,Taigu,Shanxi 030801,China;Foundation Department,Shanxi Agricultural University,Taigu,Shanxi 030801,China;Institute of Crop Science,Shanxi Academy of Agricultural Sciences,Taiyuan 030000,China)

机构地区：[1]太原师范学院地理科学学院,山西晋中030619 [2]中华人民共和国太原海关,太原030006 [3]山西农业大学经济管理学院,山西太谷030801 [4]山西农业大学植物保护学院,山西太谷030801 [5]山西农业大学基础部,山西太谷030801 [6]山西省农业科学院作物科学研究所,太原030000

出　　处：《昆虫学报》2020年第12期1473-1481,共9页Acta Entomologica Sinica

基　　金：山西省重点研发计划项目(201803D221004-8);国家现代农业食用豆产业技术体系(CARS-08-G10);山西省自然科学基金项目(201901D111230)。

摘　　要：【目的】蛋白酶抑制剂是广泛存在于植物体内的一类分子量很小的蛋白质,通过影响昆虫体内酶活性,从而抑制幼虫的生长发育。本研究旨在明确绿豆胰蛋白酶抑制剂(MBTI)对绿豆象Callosobruchus chinensis生长发育及体内酶活性的影响。【方法】采用室内人工接虫方法,测定了取食含2.0%MBTI的人工绿豆对绿豆象生长发育的抑制作用;并利用生化方法测定了绿豆象幼虫取食含不同浓度(0.5%,1.0%,1.5%和2.0%)MBTI的人工绿豆后,其体内解毒酶羧酸酯酶(CarE)和谷胱甘肽-S-转移酶(GSTs)及保护酶超氧化物歧化酶(SOD)、过氧化酶(POD)和过氧化氢酶(CAT)活性的变化。【结果】绿豆象幼虫取食含2.0%MBTI的人工绿豆后,幼虫体重和成虫羽化率均显著低于对照(取食感虫绿豆品种磨制的人工绿豆),分别只有对照的72%和55%,幼期发育历期相对延长,为对照的1.083倍,而着卵量和卵孵化率并未受到显著影响;取食MBTI含量不同的人工绿豆对各龄期幼虫体内CarE,GSTs和POD活性均有一定的促进作用,而对各龄期幼虫体内SOD和CAT活性均有一定的抑制作用,抑制作用和促进作用均随MBTI含量增加而逐渐增强,且2.0%MBTI对1龄幼虫体内CarE,GSTs和POD活性的促进作用均最强,分别比对照增加了46.5%,60.5%和67.3%,2.0%MBTI分别对4龄幼虫体内SOD活性和1龄幼虫体内CAT活性的抑制作用均最强,分别为对照的80.0%和47.6%。【结论】绿豆胰蛋白酶抑制剂对绿豆象幼虫体重和成虫羽化率均有一定抑制作用,导致幼期发育历期相对延长,同时影响其体内解毒酶和保护酶活性,从而使绿豆象幼虫不能正常生长发育。【Aim】Protease inhibitor is a low molecular weight protein widely found in plants that inhibits larval development by affecting enzyme activity in insects.This study aims to clarify the effects of mung bean trypsin inhibitor(MBTI)on the growth and development and enzyme activities in Callosobruchus chinensis.【Methods】The inhibitory effects of feeding on artificial mung bean containing 2.0%MBTI on the growth and development of C.chinensis were determined by means of artificial inoculation,and the activities of detoxifying enzymes,carboxylesterase(CarE)and glutathione-S-transferases(GSTs),and protective enzymes,superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),in the larvae fed on artificial mung bean containing different concentrations(0.5%,1.0%,1.5%and 2.0%)of MBTI were measured by biochemical methods.【Results】After the larvae of C.chinensis fed with 2.0%MBTI,the larval weight and adult emergence rate were significantly lower than those of the control(fed with the artificial mung bean made with the susceptible mung bean variety),being 72%and 55%of that of the control,respectively,and the developmental duration of immature stages was prolonged,being 1.083-fold as long as that of the control.However,MBTI had no significant effect on the number of eggs laid on beans and egg hatching rate.The CarE,GSTs and POD activities in various instar larvae were promoted by the intake of artificial mung bean with different contents of MBTI.However,the activities of SOD and CAT in various instar larvae were inhibited by the intake of artificial mung bean with different contents of MBTI.The inhibitory and promotive effects on the enzyme activities were enhanced with the increase of MBTI content.The CarE,GSTs and POD activities in the 1 st instar larvae were most strongly promoted by 2.0%MBTI,being 46.5%,60.5%and 67.3%of that in the control,respectively.MBTI at the concentration of 2.0%showed the strongest inhibitory effect on the SOD activity in the 4 th instar larvae and the CAT activity in the 1 st instar lar

关 键 词：绿豆象 绿豆 胰蛋白酶抑制剂 生长发育 解毒酶 保护酶 

分 类 号：Q968[生物学—昆虫学]