东方绢金龟雌成虫对桃树挥发物的触角电位和行为反应  

Electroantennographic and behavioral responses of female adults of Maladera orientalis(Coleoptera:Scarabaeidae)to peach tree volatiles

作　　者：张萌萌 陈宏灏[1] 王文凯[2] 陈立[3,4] ZHANG Meng-Meng;CHEN Hong-Hao;WANG Wen-Kai;CHEN Li(Institute of Plant Protection,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,China;School of Agriculture,Yangtze University,Jingzhou,Hubei 434000,China;State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China;College of Life Science,Institute of Life Science and Green Development,Hebei University,Baoding,Hebei 071002,China)

机构地区：[1]宁夏农林科学院植物保护研究所,银川750002 [2]长江大学农学院,湖北荆州434000 [3]中国科学院动物研究所,农业虫害鼠害综合治理研究国家重点实验室,北京100101 [4]河北大学生命科学学院,生命科学与绿色发展研究院,河北保定071002

出　　处：《昆虫学报》2020年第12期1482-1489,共8页Acta Entomologica Sinica

基　　金：宁夏植物病虫害防治重点实验室开放课题(NXDPL-02)。

摘　　要：【目的】东方绢金龟Maladera orientalis是我国重要的园林植物害虫。本研究通过鉴定对东方绢金龟具有引诱作用的桃树挥发物成分,为植物源引诱剂的开发奠定基础。【方法】利用顶空吸附法收集桃树枝叶挥发物,使用气质联用(GC-MS)及气相色谱触角电位(GC-EAD)鉴定桃树挥发物中对东方绢金龟雌成虫有电生理活性的物质组分;以正己烷为对照,使用触角电位(EAG)仪和Y型嗅觉仪分别测试东方绢金龟雌成虫对鉴定出的活性物质的EAG和行为反应。【结果】从桃树挥发物中共鉴定出6种活性物质,包括反-2-己烯醛、顺-3-己烯乙酸酯、反-3-己烯醇、顺-3-己烯醇、水杨酸甲酯以及1种未知化合物。当剂量为1和10μg时,东方绢金龟雌成虫对反-2-己烯醛的EAG反应值最大;当剂量为100μg时,东方绢金龟雌成虫对顺-3己烯乙酸酯的EAG反应值最小。行为反应试验结果表明,东方绢金龟雌成虫对100μg的反-3-己烯醇和顺-3-己烯醇表现出显著的趋性。【结论】桃树挥发物中的反-3-己烯醇和顺-3-己烯醇对东方绢金龟雌成虫有显著的吸引作用。【Aim】Maladera orientalis is an important garden pest in China.This study aims to identify peach tree volatile compounds attracting M.orientalis,so as to provide a theoretical basis for developing attractants of plant origin.【Methods】Leaf volatiles were collected from peach tree by dynamic headspace adsorption.The electrophysiologically active compounds for M.orientalis adults were identified from peach tree volatiles by gas chromatography-mass spectrometry(GC-MS)and coupled gas chromatography electroantennogram detection(GC-EAD)techniques.The electrophysiological and behavioral responses of female adults of M.orientalis to the identified active compounds were tested by using electroantennography(EAG)and Y-tube olfactometer,respectively,using hexane as a control.【Results】Six active compounds were identified from peach tree volatiles,including E-2-hexenal,Z-3-hexenyl acetate,E-3-hexenol,Z-3-hexenol,methyl salicylate and an unknown compound.E-2-Hexenal triggered significantly greater EAG response in female adults of M.orientalis than the other four compounds at the doses of 1 and 10μg.When the dose was increased to 100μg,Z-3-hexenyl acetate triggered significantly lower EAG response in female adults of M.orientalis than the other four compounds.In behavioral response experiments,the female adults of M.orientalis were significantly attracted to E-3-hexenol and Z-3-hexenol at the dose of 100μg.【Conclusion】Among peach tree volatile compounds,E-3-hexenol and Z-3-hexenol are significantly attractive to female adults of M.orientalis.

关 键 词：东方绢金龟 桃树挥发物 行为反应 GC-EAD 触角电位 Y型嗅觉仪 

分 类 号：Q968[生物学—昆虫学]