响应面优化微波辅助莪术总黄酮的提取工艺研究  

Optimization of extraction technology of total flavonoids from Curcuma zedoaria by response surface methodology

作　　者：唐森 王梦萱 谢济运[2,3] 吴国勇 梁健丹 张鹏[1,2] TANG Sen;WANG Meng-xuan;XIE Ji-yun;WU Guo-yong;LIANG Jian-dan;ZHANG Peng(School of Food and Biochemical Engineering,Guangxi Science&Technology Normal University,Laibin 546119;Key Laboratory for Research and Development of Characteristic Yao Medicine Resources,Guangxi Science&Technology Normal University,Laibin 546119;Scientific Research Management Department,Guangxi Science&Technology Normal University,Laibin 546119)

机构地区：[1]广西科技师范学院食品与生化工程学院,来宾546119 [2]广西科技师范学院特色瑶药资源研究与开发重点实验室,来宾546119 [3]广西科技师范学院实验实训中心,来宾546119

出　　处：《中国食品添加剂》2021年第2期42-50,共9页China Food Additives

基　　金：广西糖资源工程技术研究中心培育建设项目(桂科AD16450040);广西高校制糖工程综合技术重点实验室培育建设项目(桂教科研〔2016〕6号);2018-2020年广西本科高校食品科学与工程特色专业及实验实训教学基地(中心)建设项目(桂教高教[2018]52号);广西金秀桐木镇瑶医瑶药创新小镇培育建设项目(桂科高字[2018]299号)。

摘　　要：以莪术为原材料,采用微波辅助提取莪术中总黄酮。通过单因素试验考察微波时间、微波功率、液料比和乙醇质量分数这四个因素对莪术总黄酮提取工艺的影响,并通过响应面设计优化莪术总黄酮的最佳提取条件,确定莪术总黄酮的最佳提取工艺条件。实验结果表明,微波辅助提取莪术总黄酮的最佳工艺条件为:微波时间:5.8min、微波功率:300W、液料比:20.6∶1(mL/g)、乙醇质量分数:60%。在此实验条件下,莪术总黄酮的实际得率为22.403mg/g。This paper reports the optimization of total flavonoids from Curcuma zedoaria using response surface methodology.On the basis of single factor experiment,the influence of four factors,i.e.ratio of material to liquid,extraction time,extraction power and volume fraction of ethanol,on the extraction process of total flavonoids of zedoary turmeric was investigated,and the optimal extraction conditions of total flavonoids of zedoary turmeric were determined by response surface design.The results showed that the optimum conditions of microwave-assisted extraction of total flavonoids from curcuma zedoary were:microwave treatment time:5.8min,microwave power:300W,liquid material ratio:20.6∶1(mL/g),ethanol content:60%.Under these experimental conditions,the actual yield of total flavonoids of curcuma zedoary was 22.403mg/g.

关 键 词：莪术 总黄酮 微波提取 响应面法 

分 类 号：R284.2[医药卫生—中药学] TQ041.8[医药卫生—中医学]