CpG-ODN对^(12)C^(6+)离子照射所致胸腺损伤的防治作用  

Effect of CpG-ODN on thymus damage radiated by carbon ions

作　　者：张超 傅华 蔡建明 高福 ZHANG Chao;FU Hua;CAI Jianming;GAO Fu(Department of Radiotherapy,The 940 Hospital of Joint Logistic Support Force of PLA.,Lanzhou 730050,China;Faculty of Naval Medicine,Second Military Medical University,Shanghai 200433,China)

机构地区：[1]中国人民解放军联勤保障部队第九四〇医院放疗科,兰州730050 [2]中国人民解放军海军军医大学放射医学教研室,上海200433

出　　处：《辐射研究与辐射工艺学报》2021年第1期54-60,共7页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金项目(81501623)资助。

摘　　要：为了探讨CpG-ODN对^(12)C^(6+)离子照射所致胸腺损伤的防治作用,C57BL/6L小鼠受到5 Gy^(12)C^(6+)离子全身照射后观测其30 d存活率,计算照射后1 d和3 d胸腺指数。使用苏木精-伊红染色法评估胸腺组织病理学变化;末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法检测胸腺细胞凋亡;免疫组化法检测γ-H2AX以判断DNA双链断裂水平;免疫荧光法检测外周血中CD3+T细胞数量。实验结果显示,^(12)C^(6+)离子照射后使用CpG-ODN处理小鼠存活率提高50%,胸腺组织损伤减轻,发生凋亡和出现DNA双链断裂的细胞数量减少,外周血中CD3^(+)T细胞数量增加。这些表明CpG-ODN能够减轻^(12)C^(6+)离子照射所致的胸腺损伤,其原因可能与抑制胸腺细胞凋亡和减少细胞内DNA双链断裂有关。To investigate the ameliorative effect of CpG-ODN on thymus damage after carbon ion radiation(CIR),C57BL/6L mice were irradiated with 5 Gy of CIR,and 30-day survival was observed.The index of the thymus was calculated and the degree of thymus damage was evaluated by histological examination.Apoptosis was detected by terminal dexynucleotidyl transferase-mediated dUTP nick and labeling(TUNEL)assay and doublestrand break(DSB)was detected byγ-H2AX immunohistochemistry.The number of T cells in the peripheral blood was examined by CD3 antibody labeling.The results showed that CpG-ODN increased mice survival by 50%,obviously ameliorated thymus damage,reduced the number ofγ-H2AX foci and TUNEL-positive cells of the thymus,and increased the number of CD3^(+)T cells in peripheral blood after CIR.This indicated that CpG-ODN could ameliorate thymus damage induced by CIR.These findings might be related to the inhibition of DSB and apoptosis.

关 键 词：^(12)C^(6+)离子照射 CPG-ODN 胸腺 凋亡 DNA双链断裂 

分 类 号：R3[医药卫生—基础医学]