PBMCs中NS5ATP9、IL-12p40、IRAK-1的mRNA水平联合检测对早期胃癌的诊断价值  

Diagnostic value of NS5ATP9,IL-12p40,IRAK-1 mRNA levels in PBMCs for early gastric cancer

作　　者：赵勇 曾玮荣 曾俊峰 ZHAO Yong;ZENG Weirong;ZENG Junfeng(Department of Clinical Laboratory,Wuhan Third Hospital,Wuhan,Hubei 430060,China)

机构地区：[1]武汉市第三医院检验科,湖北武汉430060

出　　处：《国际检验医学杂志》2021年第4期465-468,472,共5页International Journal of Laboratory Medicine

摘　　要：目的探讨外周血单个核细胞(PBMCs)中非结构蛋白5A反式激活蛋白9(NS5ATP9)、白细胞介素-12p40(IL-12p40)、白细胞介素1受体相关激酶1(IRAK-1)的mRNA水平联合检测对早期胃癌的诊断价值。方法将2017年11月至2019年11月该院收治的早期胃癌患者112例作为早期胃癌组,胃部良性疾病患者112例作为良性组,体检健康者112例作为对照组。从3组受试人员外周血标本提取PBMCs后通过实时荧光定量PCR检测NS5ATP9、IL-12p40、IRAK-1的mRNA水平。用受试者工作特征曲线(ROC曲线)的曲线下面积(AUC)分析NS5ATP9、IL-12p40、IRAK-1的mRNA水平在早期胃癌中的诊断意义。结果早期胃癌患者PBMCs中NS5ATP9、IL-12p40、IRAK-1的mRNA水平与胃部良性疾病患者和体检健康者比较,差异均有统计学意义(P<0.05)。PBMCs中NS5ATP9、IL-12p40、IRAK-1 mRNA水平用于鉴别早期胃癌患者与胃部良性疾病患者的灵敏度分别为91%、87%、93%,特异度分别为91%、88%、92%。PBMCs中NS5ATP9、IL-12p40、IRAK-1 mRNA水平用于区分早期胃癌患者与体检健康者的灵敏度分别为96%、92%、95%,特异度分别为94%、91%、95%。PBMCs中NS5ATP9、IL-12p40、IRAK-1 mRNA联合检测用于早期胃癌诊断的灵敏度为92.86%,特异度为93.30%,准确度为93.15%。结论PBMCs中的NS5ATP9、IL-12p40、IRAK-1 mRNA是早期胃癌的潜在标志物。Objective To explore the diagnostic value of the combined detection of nonstructural protein 5A transactivator 9(NS5ATP9),interleukin-12p40(IL-12p40)and interleukin-1 receptor-associated kinase-1(IRAK-1)mRNA levels in Peripheral blood mononuclear cells(PBMCs)for early gastric cancer.Methods A total of 112 patients with early gastric cancer admitted in the hospital from October 2017 to October 2019 were enrolled in the study as early gastric cancer group,during the same period 112 patients with benign gastric disease were enrolled as benign group,and 112 healthy people as the control group.PBMCs were extracted from the people of the 3 groups and the mRNA levels of NS5ATP9,IL-12p40,and IRAK-1 in those PBMCs samples were detected by real-time fluorescent quantitative PCR.The area under curve(AUC)of receiver operating characteristic curve(ROC)was used to analyze the diagnostic value of NS5ATP9,IL-12p40,IRAK-1 mRNA levels in PBMCs for early gastric cancer.Results The mRNA levels of NS5ATP9,IL-12p40 and IRAK-1 in PBMCs of patients with early gastric cancer were significantly higher than those of patients with benign gastric diseases and healthy people(P<0.05).The sensitivity of NS5ATP9,IL-12p40,and IRAK-1 mRNA levels in PBMCs to distinguish patients with early gastric cancer from patients with benign gastric diseases were 91%,87%,and 93%,and the specificities were 91%,88%,and 92%,respectively.The sensitivity of NS5ATP9,IL-12p40,and IRAK-1 mRNA levels in PBMCs used to distinguish early gastric cancer patients from healthy subjects were 96%,92%,95%,and specificity were 94%,91%,95%,respectively.The sensitivity of the combined detection of NS5ATP9,IL-12p40,and IRAK-1 mRNA in PBMCs for the diagnosis of early gastric cancer was 92.86%,the specificity was 93.30%,and the accuracy was 93.15%.Conclusion NS5ATP9,IL-12p40 and IRAK-1 mRNA in PBMCs are potential markers of early gastric cancer.

关 键 词：外周血单个核细胞 非结构蛋白5A反式激活蛋白9 白细胞介素-12p40 白细胞介素-1受体相关激酶-1 胃癌 诊断 

分 类 号：R446.19[医药卫生—诊断学]